LOCUS       sequence-80950-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Park JE, Heo I, Tian Y, Simanshu DK, Chang H, Jee D, Patel DJ, Kim 
            VN
  TITLE     Dicer recognizes the 5' end of RNA for efficient and accurate 
            processing.
  JOURNAL   Nature. 2011 Jul 13;475(7355):201-5. doi: 10.1038/nature10198.
  PUBMED    21753850
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-13- 13"; volume: "475"; issue: "7355"; 
            pages: "201-5"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mut...
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcccacca tggactacaa ggacgacgat gacaagaagc
      121 ttatggcagg cctgcagctc atgacccctg cttcctcacc aatgggtcct ttctttggac
      181 tgccatggca acaagaagca attcatgata acatttatac gccaagaaaa tatcaggttg
      241 aactgcttga agcagctctg gatcataata ccatcgtctg tttaaacact ggctcaggga
      301 agacatttat tgcagtacta ctcactaaag agctgtccta tcagatcagg ggagacttca
      361 gcagaaatgg aaaaaggacg gtgttcttgg tcaactctgc aaaccaggtt gctcaacaag
      421 tgtcagctgt cagaactcat tcagatctca aggttgggga atactcaaac ctagaagtaa
      481 atgcatcttg gacaaaagag agatggaacc aagagtttac taagcaccag gttctcatta
      541 tgacttgcta tgtcgccttg aatgttttga aaaatggtta cttatcactg tcagacatta
      601 accttttggt gtttgatgag tgtcatcttg caatcctaga ccacccctat cgagaaatta
      661 tgaagctctg tgaaaattgt ccatcatgtc ctcgcatttt gggactaact gcttccattt
      721 taaatgggaa atgtgatcca gaggaattgg aagaaaagat tcagaaacta gagaaaattc
      781 ttaagagtaa tgctgaaact gcaactgacc tggtggtctt agacaggtat acttctcagc
      841 catgtgagat tgtggtggat tgtggaccat ttactgacag aagtgggctt tatgaaagac
      901 tgctgatgga attagaagaa gcacttaa
//
