LOCUS       sequence-80952-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses hygromycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  TITLE     empty vector
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 hygro
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             95..328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     410..426
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     411..427
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat
       61 aaatactgaa taataagatg acatgaacta ctactgctag agattttcca cactgactaa
      121 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
      181 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
      241 tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt
      301 cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga
      361 tctacagctg ccttgtaagt cattggtctt aaaggtaccg ggccccccct cgaggtcgac
      421 ggtatcgata gatcccggtc ggcatctact ctattccttt gccctcggac gagtgctggg
      481 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
      541 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
      601 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
      661 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
      721 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
      781 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
      841 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
      901 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
      961 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1021 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1081 cccg
//
