LOCUS       sequence-80955-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS2113
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             100..111
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     509..529
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(640..661)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
       61 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
      121 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
      181 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
      241 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
      301 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
      361 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
      421 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
      481 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
      541 accagtcaat ctttcacaaa ttttgtaatc cagaggttga gcgatcgcgg ccgcttactt
      601 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      661 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
      721 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      781 ctgcacgctg ccgtcctcga tgttgtggcg ggtcttgaag ttcaccttga tgccgttctt
      841 ctgcttgtcg gcggtgatat agaccttgtg gctgttgtag ttgtactcca gcttgtgccc
      901 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcacc
//
