LOCUS       sequence-80958-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expresses FLAG/HA-tagged MOV10 K530A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pFRT/FLAG/HA MOV10 K530A
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..49)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    102..126
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    528..539
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 actcgagcgt agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg
       61 ggccctctag acttgagcgg ccgccactgt gctggatatc aaccactttg tacaagaaag
      121 ctgggtctag atatctcgag tgcggccgct cagagctcat tcctccactc tggctccact
      181 tgcagagaca ggcccccttc accctcccgc tcctggggga ggtagtcatg gctgtggggc
      241 cctgaggtag aggggctgag cttgctcaga ccttgcagta aattctgtcc ctgttgcagg
      301 tccagtttgg cagggaaggg acacccggta taccctccgt tttctttaca gaactccagg
      361 aatactttcc agtcagggtc atggcccagg agaagggggt tccccacgat gatgagcagg
      421 gccttggccc gggtcacagc tacattgaac ctcttggggt tcttaaggaa acccagatta
      481 aagtccagat ccagctgcac aaagctctgg ctgcttcgca cggtggagat gaggatgacg
      541 cttcgttctt ggccttggaa ttcttctact gaacccacct tcaagtcctt gatgtcatcc
      601 agtcctcgaa gctccctgtc aagtttggtg atgcagtaac ggattttctc cacctgtttc
      661 cggtacgggg agatgacgcc cacacttcga gggctcaggc gagctttgcc cttcttggag
      721 gagggggcca ggagcagctt caggtaggaa gtcactgtgg cagcctcttc agggttgaag
      781 aaggatgggc tgttgccttc acgctcatct ttgcccatta cgccgtgaaa gatgatggga
      841 aagccctgtc gaggtaggcc cgccc
//
