LOCUS       sequence-80959-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expresses FLAG/HA-tagged MOV10 D645N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Gregersen LH, Schueler M, Munschauer M, Mastrobuoni G, Chen W, Kempa
            S, Dieterich C, Landthaler M
  TITLE     MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target 
            degradation by translocation along 3' UTRs.
  JOURNAL   Mol Cell. 2014 May 22;54(4):573-85. doi: 
            10.1016/j.molcel.2014.03.017. Epub 2014 Apr 10.
  PUBMED    24726324
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.017"; journalName: "Mol Cell"; date: 
            "2014-05-22- 22"; volume: "54"; issue: "4"; pages: "573-85"
COMMENT     Sequence Label: pFRT/FLAG/HA MOV10 D645N
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..52)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    531..542
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 tagactcgag cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc
       61 gcgggccctc tagacttgag cggccgccac tgtgctggat atcaaccact ttgtacaaga
      121 aagctgggtc tagatatctc gagtgcggcc gctcagagct cattcctcca ctctggctcc
      181 acttgcagag acaggccccc ttcaccctcc cgctcctggg ggaggtagtc atggctgtgg
      241 ggccctgagg tagaggggct gagcttgctc agaccttgca gtaaattctg tccctgttgc
      301 aggtccagtt tggcagggaa gggacacccg gtataccctc cgttttcttt acagaactcc
      361 aggaatactt tccagtcagg gtcatggccc aggagaaggg ggttccccac gatgatgagc
      421 agggccttgg cccgggtcac agctacattg aacctcttgg ggttcttaag gaaacccaga
      481 ttaaagtcca gatccagctg cacaaagctc tggctgcttc gcacggtgga gatgaggatg
      541 acgcttcgtt cttggccttg gaattcttct actgaaccca ccttcaagtc cttgatgtca
      601 tccagtcctc gaagctccct gtcaagtttg gtgatgcagt aacggatttt ctccacctgt
      661 ttccggtacg gggagatgac gcccacactt cgagggctca ggcgagcttt gcccttcttg
      721 gaggaggggg ccaggagcag cttcaggtag gaagtcactg tggcagcctc ttcagggttg
      781 aagaaggatg ggctgttgcc ttcacgctca tctttgccca ttacgccgtg aaagatgatg
      841 ggaaagccct gtcgaggtag gcccgcccag cggcagaagc gttctcgatc cacgacatca
      901 gcacaggcct gcagctccc
//
