LOCUS       sequence-8096-ex        5348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1097
FEATURES             Location/Qualifiers
     source          1..5348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..1039
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(356..384)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     979..999
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1071..1157
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1846..2508)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2509..3278
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4561..5094)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5283..5288,5289..5305,5306..5311))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggattgg ctttactaag ctgatccggt ggatgacctt ttgaatgacc
       61 tttaatagat tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa
      121 aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac cggatctaga
      181 tatcgattaa ttaagtcgac atctagagtt taaactttaa ttaagcccgg gctgcagccc
      241 gggggatcca ctaggttaac gcatgcgggc ccggtaccag gcctagatag atagagagag
      301 agagagacta gtggaggaag aaaaaatgcg taaaggagaa gaacttttca ctggagttgt
      361 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      421 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      481 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      541 gagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
      601 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      661 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      721 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      781 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      841 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      901 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      961 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     1021 catggatgaa ctatacaaat gagctctaca aataatgaat tcccaggcat caaataaaac
     1081 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1141 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     1201 ggtggcgggc aggacgcccg ccataaactg ccagggaatt ccaactgagc gccggtcgct
     1261 accattacca acttgtctgg tgtcaaaaat aataggattt tgttaaaatt cgcgttaaat
     1321 ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg gcagggcggg
     1381 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa
     1441 gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc
     1501 agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac
     1561 cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
     1621 cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac
     1681 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat
     1741 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg gctacagccg
     1801 atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc ggcgcggcag
     1861 cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg gctcatcggg
     1921 catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca aggctggcag
     1981 gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg
     2041 tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat gccccaacag
     2101 cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg acaggcgcaa
     2161 ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg acacggtgcc
     2221 ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct tgactgcgta
     2281 ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc tgaccaccac
     2341 ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg ccgtgggttt
     2401 cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca cccagtgacc
     2461 gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc ttatctccat
     2521 gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg
     2581 cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg
     2641 caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg
     2701 ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg
     2761 cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc
     2821 ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc
     2881 ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccttc
     2941 gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg
     3001 cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc
     3061 gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc
     3121 cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca
     3181 atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt
     3241 gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc
     3301 cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta
     3361 gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact
     3421 caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga
     3481 caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa gaagctggcg
     3541 aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga gacgcccaac
     3601 gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac cgatgaagcg
     3661 atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt gttggcggtc
     3721 gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga acagcaggcg
     3781 gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga tcgcatcgtg
     3841 acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg
     3901 acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca gatgacccgc
     3961 gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg catcgagggc
     4021 agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg gccaccagtg
     4081 ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc gcagggattg
     4141 gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg
     4201 acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg agcgcgtgag
     4261 atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga gcgcctgaag
     4321 cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc cgccgcgatc
     4381 atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg ccagaaacag
     4441 gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca cctggcggcg
     4501 ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg
     4561 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
     4621 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
     4681 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct
     4741 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
     4801 cctgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
     4861 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
     4921 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
     4981 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
     5041 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc
     5101 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     5161 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     5221 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     5281 acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg
     5341 gtgtgcgt
//
