LOCUS       sequence-80963-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express DsRed monomer-tagged hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pDsRed-monomer-hAIM2
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..781)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(364..383)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      778..787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagggcccgt acggccgact agtaggccta ttatttttga caccagacaa gttggtaatg
       61 gtagcgaccg gcgctcagtt ggaattctag agtcgcggcc gctctactgg gagccggagt
      121 ggcgggcctc ggcgtgctcg tactgctcca ccacggtgta gtcctcgttg tggttggtga
      181 tgtccagctt ggagtccacg tagtggttgc cgggcagctg cacgggcttc ttggccttgt
      241 acacggtctt gaagtcgcag gtgtagtggc cgccgtcctt cagcttcagg gcgtgggaga
      301 tctcgccctt cagcacgccg tcctgggggt acagcttctc ggtggagggc tcccagccgg
      361 cagtcttctt ctgcattacg gggccgtcgg cggggaagtt cacgcccttg aacttcacct
      421 tgtagatgaa ggtgccgtcc tgcagggagg agtcctgctg cacctccacc acgccgccgt
      481 cctcgaagtt catggagcgc tcccaggtga agccctcggg gaaggacagc ttcatgtagt
      541 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtactggaac tggggggaca
      601 ggatgtccca ggcgaagggc agggggccgc ccttggtcac ctgcagcttg gcggtctggg
      661 tgccctcgta gggcttgccc tcgccctcgc cctcgatctc gaagtagtgg ccgttcacgg
      721 agccctccat gcgcaccttg aactgcatga actccttgat gacgtcctcg gtgttgtcca
      781 tggtggcgac cggtacccgg ggatcctctg tttttttttt ggccttaata acctttatgg
      841 tgctatgaac tccagatgtc agctgtagtt tttctccatt ttttgacagt gtgaagaatg
      901 taagtcgaac cttatctcct tccttacatt tcattgtgtc ctcgtttcta acccc
//
