LOCUS       sequence-80965-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-GST
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..716)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(83..105)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga cttaacgcgg aaccagatcc
       61 gattttggag gatggtcgcc accaccaaac gtggcttgcc agccctgcaa aggccatgct
      121 atatacttgc tggatttcaa gtacttatca atttgtggga tagcttcaat acgtttttta
      181 aaacaaacta attttgggaa cgcatccagg cacattgggt ccatgtataa aacaacatca
      241 agagcgtcat acaacatgaa gtcaggatgg gttacatgat caccatttaa atatgtttta
      301 tgacataaac gatcttcgaa cattttcagc atttcaggta gcttgctaag aaaatcaact
      361 ttgagagttt caaagtcttt actatatgca attctcgaaa caccgtatct aatatccaaa
      421 accgctcctt caagcattga aatctctgca cgctcttttg gacaaccacc caacatgttg
      481 tgcttgtcag ctatataacg tatgatggcc atagactgtg ttaatttaac atcaccatca
      541 atataataag gaagattggg aaactccaaa cccaattcaa actttttgtt tcgccattta
      601 tcaccttcat cgcgctcata caaatgctct tcatattttt cttcaagata ttccaaaaga
      661 agtcgagtgg gttgcacaag gcccttaatt ttccaataac ctagtatagg ggacatctcg
      721 agtgtttttt ttttggcctt aataaccttt atggtgctat gaactccaga tgtcagctgt
      781 agtttttctc cattttttga cagtgtgaag aatgtaagtc gaaccttatc tccttcctta
      841 cattt
//
