LOCUS       sequence-80968-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged HIN domain of mAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-mAIM2-deltaPYD-GST
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(61..714)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(81..103)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgact taacgcggaa ccagatccga
       61 ttttggagga tggtcgccac caccaaacgt ggcttgccag ccctgcaaag gccatgctat
      121 atacttgctg gatttcaagt acttatcaat ttgtgggata gcttcaatac gttttttaaa
      181 acaaactaat tttgggaacg catccaggca cattgggtcc atgtataaaa caacatcaag
      241 agcgtcatac aacatgaagt caggatgggt tacatgatca ccatttaaat atgttttatg
      301 acataaacga tcttcgaaca ttttcagcat ttcaggtagc ttgctaagaa aatcaacttt
      361 gagagtttca aagtctttac tatatgcaat tctcgaaaca ccgtatctaa tatccaaaac
      421 cgctccttca agcattgaaa tctctgcacg ctcttttgga caaccaccca acatgttgtg
      481 cttgtcagct atataacgta tgatggccat agactgtgtt aatttaacat caccatcaat
      541 ataataagga agattgggaa actccaaacc caattcaaac tttttgtttc gccatttatc
      601 accttcatcg cgctcataca aatgctcttc atatttttct tcaagatatt ccaaaagaag
      661 tcgagtgggt tgcacaaggc ccttaatttt ccaataacct agtatagggg acatctcgag
      721 ctccacactt ttcatgtcag tttttggctt tgcagcctta ataaccttaa aaaagctaca
      781 aggtccagat ttcaactgaa ttttcactcc attttttgac acctcaaaga acgtgagtct
      841 aatcttgtct ccttcctcgc actttgtttt gcttgggttt cccagcacca acacctccat
      901 tgtccctgtt ttatcatgta taccatacag tactctatct ttttcctctg
//
