LOCUS       sequence-80969-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GST-tagged PYD of hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pET21a-hAIM2-PYD-GST
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(64..717)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(84..106)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg acttaacgcg gaaccagatc
       61 cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca aaggccatgc
      121 tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa tacgtttttt
      181 aaaacaaact aattttggga acgcatccag gcacattggg tccatgtata aaacaacatc
      241 aagagcgtca tacaacatga agtcaggatg ggttacatga tcaccattta aatatgtttt
      301 atgacataaa cgatcttcga acattttcag catttcaggt agcttgctaa gaaaatcaac
      361 tttgagagtt tcaaagtctt tactatatgc aattctcgaa acaccgtatc taatatccaa
      421 aaccgctcct tcaagcattg aaatctctgc acgctctttt ggacaaccac ccaacatgtt
      481 gtgcttgtca gctatataac gtatgatggc catagactgt gttaatttaa catcaccatc
      541 aatataataa ggaagattgg gaaactccaa acccaattca aactttttgt ttcgccattt
      601 atcaccttca tcgcgctcat acaaatgctc ttcatatttt tcttcaagat attccaaaag
      661 aagtcgagtg ggttgcacaa ggcccttaat tttccaataa cctagtatag gggacatctc
      721 gagtggcttt ggttttgtta ccgatttgta ttgcttatca actttctcct tctcctcctg
      781 aagacgtttt gccaaaagca tataattcaa cttctgaaaa atacgaatgg tcttcatcac
      841 tgcagacacc gccccagcat tttgaatcat caaggtagct acttgtattc tgtttgcagt
      901 atgtagtttg cctgtggcaa tattaaactc gtctgaaaga aagaacttaa acctatccag
      961 t
//
