LOCUS       sequence-80970-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  self-inactivating lentiviral plasmid for co-expression of your gene 
            of interest and dTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  TITLE     pHIV-dTomato
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHIV-dTomato
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(118..126,127..129,130..704)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(291..310)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     520..537
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat
       61 gcgcaccttg aagcgcatga actctttgat gacctcctcg cccttgctca ctgacatggt
      121 tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
      181 ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgtaccga ggccccagat
      241 cagatcccat acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg
      301 aggagagcca tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg
      361 ccgcctttgc aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgtcgccag
      421 gtggggggtt ccgctgcctg caaagggtcg ctacagacgt tgtttgtctt caagaagctt
      481 ccagaggaac tgcttccttc acgacattca acagaccttg cattcctttg gcgagagggg
      541 aaagacccct aggaatgctc gtcaagaaga cagggccagg tttccgggcc ctcacattgc
      601 caaaagacgg caatatggtg gaaaataaca tatagacaaa cgcacaccgg ccttattcca
      661 agcggcttcg gccagtaacg ttaggggggg gggagggaga ggggcggatc ctagcccggg
      721 tactctagaa tag
//
