LOCUS       sequence-80972-e        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and an EGFP targeting synthetic 
            single-guide RNA (sgRNA) element from U6 promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, 
            Heckl D, Ebert BL, Root DE, Doench JG, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout screening in human cells.
  JOURNAL   Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. 
            Epub 2013 Dec 12.
  PUBMED    24336571
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1247005"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "84-7"
COMMENT     Sequence Label: lentiCRISPR - EGFP sgRNA 1
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtcgccggcc tgcttcagca ggctgaagtt ggtggcgccg ctgccgctag ctttcttttt
       61 cttagcttga ccagctttct tagtagcagc aggacgcttg tcgcctccca gctgagacag
      121 gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac
      181 ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc
      241 aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat
      301 gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag
      361 gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg
      421 ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca gcttctcata
      481 gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt cgtttccctt
      541 ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct cgaacaggga
      601 gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct tggcttccag
      661 aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc
      721 tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga
      781 ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt ccttctttct
      841 ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga agccgcctgt
      901 ctgcacctcg gtctttttca cgatattcac ttggggcatg ctcagcactt tccgcacggt
      961 ggcaaaatcc cggcccttat cccacacgat ctccccggtt tcgccgtttg tctcgatcag
     1021 aggcc
//
