LOCUS       sequence-80973-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli fis ORF cloned into pBAD24 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Malagon F
  TITLE     RNase III is required for localization to the nucleoid of the 5' 
            pre-rRNA leader and for optimal induction of rRNA synthesis in E. 
            coli.
  JOURNAL   RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 
            Jul 26.
  PUBMED    23893733
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038588.113"; journalName: "RNA"; date: "2013-09"; 
            volume: "19"; issue: "9"; pages: "1200-7"
COMMENT     Sequence Label: pBAD24-fis
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(509..526)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      679..765
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttgggctagc aggaggaatt cgccaatgtt
       61 cgaacaacgc gtaaattctg acgtactgac cgtttctacc gttaactctc aggatcaggt
      121 aacccaaaaa cccctgcgtg actcggttaa acaggcactg aagaactatt ttgctcaact
      181 gaatggtcag gatgtgaatg acctctatga gctggtactg gctgaagtag aacagcccct
      241 gttggacatg gtgatgcaat acacccgtgg taaccagacc cgtgctgcgc tgatgatggg
      301 catcaaccgt ggtacgctgc gtaaaaaatt gaaaaaatac ggcatgaact aattcaggtt
      361 agctaaatgc ttgattaaaa aggcgctact cggcatgggg aagcgccttt tttataggtg
      421 tcacaaaggg agtgacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct
      481 gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg
      541 gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc
      601 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
      661 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      721 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
      781 ttgaacgttg cgaagcaacg gccggaa
//
