LOCUS       sequence-80974-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Superfolder GFP ORF cloned into pBAD24 for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Malagon F
  TITLE     RNase III is required for localization to the nucleoid of the 5' 
            pre-rRNA leader and for optimal induction of rRNA synthesis in E. 
            coli.
  JOURNAL   RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 
            Jul 26.
  PUBMED    23893733
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038588.113"; journalName: "RNA"; date: "2013-09"; 
            volume: "19"; issue: "9"; pages: "1200-7"
COMMENT     Sequence Label: pBAD24-sfGFPx1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..774
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(825..842)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcgcca
       61 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
      121 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
      181 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
      241 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
      301 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
      361 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
      421 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
      481 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
      541 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
      601 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
      661 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
      721 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgatga
      781 cccgggaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
      841 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
      901 ccacctgacc cc
//
