LOCUS       sequence-80975-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It
            contains additional aminoacids in the N and C terminus (polilinker 
            sequences for cloning proteins in frame with GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Malagon F
  TITLE     RNase III is required for localization to the nucleoid of the 5' 
            pre-rRNA leader and for optimal induction of rRNA synthesis in E. 
            coli.
  JOURNAL   RNA. 2013 Sep;19(9):1200-7. doi: 10.1261/rna.038588.113. Epub 2013 
            Jul 26.
  PUBMED    23893733
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038588.113"; journalName: "RNA"; date: "2013-09"; 
            volume: "19"; issue: "9"; pages: "1200-7"
COMMENT     Sequence Label: pBAD24-sfGFPx2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..784
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLECNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSIDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(849..866)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 actctctact gtttctccat acccgttttt ttgggctagc aggaggaatt cgccaatggc
       61 tcgagcccgg gcgcgtaaag gcgaagagct gttcactggt gtcgtcccca ttctggtgga
      121 actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc
      181 aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg
      241 gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca
      301 tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac
      361 gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga
      421 taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct
      481 gggccataag ctggaatgca attttaacag ccacaatgtt tacatcaccg ccgataaaca
      541 aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca
      601 gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga
      661 caatcactat ctgagcacgc aaagcgttct gtctatagat ccgaacgaga aacgcgatca
      721 tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta
      781 caaaggtcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag
      841 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
      901 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactca
//
