LOCUS       sequence-80982-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of superfolder GFP for protein purification 
            (Intein-chiting binding domain fussion, New England Biolabs pTXB1 
            backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-sfGFP
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..737
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actttaagaa ggagatatac ataatgcgta aaggcgaaga gctgttcact ggtgtcgtcc
       61 ctattctggt ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg
      121 gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac
      181 tgccggtacc ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc
      241 gttatccgga ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg
      301 tgcaggaacg cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga
      361 aatttgaagg cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagaag
      421 acggcaatat cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca
      481 ccgccgataa acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg
      541 atggcagcgt gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg
      601 ttctgctgcc agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg
      661 agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta
      721 tggatgaact gtacaaatgc atcacgggag atgcactagt tgccctaccc gagggcgagt
      781 cggtacgcat cgccgacatc gtgccgggtg cgcggcccaa cagtgacaac gccatcgacc
      841 tgaaagtcct tgaccggcat ggcaatcccg tgctcgccga ccggctgttc cactccggcg
      901 agcatccggt gtacacggtg cgtacggtcg aaggtctgcg tgtgacgggc accgcgaacc
      961 acccgttgtt gtgtttggtc
//
