LOCUS       sequence-80987-e         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of E. coli FIS for protein purification (Intein-chiting 
            binding domain fussion, New England Biolabs pTXB1 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-FIS
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             317..910
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 actttaagaa ggagatatac atatgttcga acaacgcgta aattctgacg tactgaccgt
       61 ttctaccgtt aactctcagg atcaggtaac ccaaaaaccc ctgcgtgact cggttaaaca
      121 ggcactgaag aactattttg ctcaactgaa tggtcaggat gtgaatgacc tctatgagct
      181 ggtactggct gaagtagaac agcccctgtt ggacatggtg atgcaataca cccgtggtaa
      241 ccagacccgt gctgcgctga tgatgggcat caaccgtggt acgctgcgta aaaaattgaa
      301 aaaatacggc atgaactgca tcacgggaga tgcactagtt gccctacccg agggcgagtc
      361 ggtacgcatc gccgacatcg tgccgggtgc gcggcccaac agtgacaacg ccatcgacct
      421 gaaagtcctt gaccggcatg gcaatcccgt gctcgccgac cggctgttcc actccggcga
      481 gcatccggtg tacacggtgc gtacggtcga aggtctgcgt gtgacgggca ccgcgaacca
      541 cccgttgttg tgtttggtcg acgtcgccgg ggtgccgacc ctgctgtgga agctgatcga
      601 cgaaatcaag ccgggcgatt acgcggtgat tcaacgcagc gcattcagcg tcgactgtgc
      661 aggttttgcc cgcgggaaac ccgaatttgc gcccacaacc tacacagtcg gcgtccctgg
      721 actggtgcgt ttcttggaag cacaccaccg agacccggac gcccaagcta tcgccgacga
      781 gctgaccgac gggcggttct actacgcgaa agtcgccagt gtcaccgacg ccggcgtgca
      841 gccggtgtat agccttcgtg tcgacacggc agaccacgcg tttatcacga acgggttcgt
      901 cagccacgct actggcctca ccggtctgaa ctcaggcctc acgacaaatc ctggtgtatc
      961 cgcttggcag g
//
