LOCUS       sequence-80988-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of bacteriophage lambda CI for protein purification 
            (Intein-chiting binding domain fussion, New England Biolabs pTXB1 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-CI
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..733
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 actttaagaa ggagatatac atatgagcac aaaaaagaaa ccattaacac aagagcagct
       61 tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc
      121 ccaggaatct gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa
      181 tggcatcaat gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag
      241 cgttgaagaa tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag
      301 tatgcagccg tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg
      361 gatgttctca cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac
      421 aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc
      481 accaacaggc tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca
      541 ggctgttgag ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa
      601 gaaactgatc agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat
      661 gatcccatgc aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga
      721 agagacgttt ggctgcatca cgggagatgc actagttgcc ctacccgagg gcgagtcggt
      781 acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa
      841 agtccttgac cggcatggca atcccgtgct cgccgaccgg ctgttccact ccggcgagca
      901 tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg acgggcaccg cgaacc
//
