LOCUS       sequence-80991-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of bacteriophage lambda CII for protein purification 
            (Intein-chiting binding domain fussion, New England Biolabs pTXB1 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Plamids for protein purification in E. coli of superfolder GFP, 
            CRP/CAP (E. coli), FIS (E. coli), CI (lambda phage) and CII (lambda 
            phage)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTXB1-CII
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(261..295)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             314..907
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 actttaagaa ggagatatac atatggttcg tgcaaacaaa cgcaacgagg ctctacgaat
       61 cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact gagaagacag cggaagctgt
      121 gggcgttgat aagtcgcaga tcagcaggtg gaagagggac tggattccaa agttctcaat
      181 gctgcttgct gttcttgaat ggggggtcgt tgacgacgac atggctcgat tggcgcgaca
      241 agttgctgcg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      301 ccagatggag ttctgcatca cgggagatgc actagttgcc ctacccgagg gcgagtcggt
      361 acgcatcgcc gacatcgtgc cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa
      421 agtccttgac cggcatggca atcccgtgct cgccgaccgg ctgttccact ccggcgagca
      481 tccggtgtac acggtgcgta cggtcgaagg tctgcgtgtg acgggcaccg cgaaccaccc
      541 gttgttgtgt ttggtcgacg tcgccggggt gccgaccctg ctgtggaagc tgatcgacga
      601 aatcaagccg ggcgattacg cggtgattca acgcagcgca ttcagcgtcg actgtgcagg
      661 ttttgcccgc gggaaacccg aatttgcgcc cacaacctac acagtcggcg tccctggact
      721 ggtgcgtttc ttggaagcac accaccgaga cccggacgcc caagctatcg ccgacgagct
      781 gaccgacggg cggttctact acgcgaaagt cgccagtgtc accgacgccg gcgtgcagcc
      841 ggtgtatagc cttcgtgtcg acacggcaga ccacgcgttt atcacgaacg ggttcgtcag
      901 ccacgctact gg
//
