LOCUS       sequence-80999-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9-nickase in mammalian cells and zygotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Fujii W, Onuma A, Sugiura K, Naito K
  TITLE     Efficient generation of genome-modified mice via offset-nicking by 
            CRISPR/Cas system.
  JOURNAL   Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4.
            doi: 10.1016/j.bbrc.2014.01.141.
  PUBMED    24491566
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 
            10.1016/j.bbrc.2014.01.141."
COMMENT     Sequence Label: pCAG-T3-hCasD10A-pA
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        83..101
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             195..215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
       61 atcattttgg caaagaattc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc
      121 accgcggtgg cggccgctct agcccgggcg gatccaccat ggattacaag gatgacgacg
      181 ataagatcat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg
      241 acaagaagta ctccattggg ctcgccatcg gcacaaacag cgtcggctgg gccgtcatta
      301 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
      361 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca
      421 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
      481 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
      541 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
      601 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
      661 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
      721 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg
      781 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca
      841 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tccc
//
