LOCUS       sequence-81000-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and an EGFP targeting synthetic 
            single-guide RNA (sgRNA) element from U6 promoter. Lentiviral 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, 
            Heckl D, Ebert BL, Root DE, Doench JG, Zhang F
  TITLE     Genome-scale CRISPR-Cas9 knockout screening in human cells.
  JOURNAL   Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. 
            Epub 2013 Dec 12.
  PUBMED    24336571
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1247005"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "84-7"
COMMENT     Sequence Label: lentiCRISPR - EGFP sgRNA 4
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..100)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgtcgccggc ctgcttcagc aggctgaagt tggtggcgcc gctgccgcta gctttctttt
       61 tcttagcttg accagctttc ttagtagcag caggacgctt gtcgcctccc agctgagaca
      121 ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg gcgtccagca
      181 cctctttggt gctggtgtac ctcttccggt cgatggtggt gtcaaagtac ttgaaggcgg
      241 caggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc tgctctctga
      301 tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta gcgtcggcca
      361 ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg tagtgcttgt
      421 gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc agcttctcat
      481 agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt tcgtttccct
      541 tctgcagttc gccggcagag gccagcattc tcttccggcc gttttccagc tcgaacaggg
      601 agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc ttggcttcca
      661 gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc cccagcagct
      721 ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc accagcacag
      781 aataggccac ggtggggctg tcgaagccgc cgtacttctt agggtcccag tccttctttc
      841 tggcgatcag cttatcgctg ttcctcttgg gcaggataga ctctttgctg aagccgcctg
      901 tctgcacctc ggtctttttc acgatattca cttggggcat gctcagcact ttccgcacgg
      961 tggcaaaatc ccggccctta tcccacacga tctccccggt ttcgccgttt gtctcgatca
     1021 gaggccgctt ccggatctcg ccgttggcca gggtaatctc ggtct
//
