LOCUS       sequence-81001-e         144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9-nickase in mammalian cells and zygotes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 144)
  AUTHORS   Fujii W, Onuma A, Sugiura K, Naito K
  TITLE     Efficient generation of genome-modified mice via offset-nicking by 
            CRISPR/Cas system.
  JOURNAL   Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4.
            doi: 10.1016/j.bbrc.2014.01.141.
  PUBMED    24491566
REFERENCE   2  (bases 1 to 144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 
            10.1016/j.bbrc.2014.01.141."
COMMENT     Sequence Label: pCAG-T3-hCasD10A-pA
FEATURES             Location/Qualifiers
     source          1..144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttccgtacac
       61 ttgcatgcta cctcgagttt tttttttttt tttttttttt tttttttttt tttttttttt
      121 tttttttttt tttttttttt tttt
//
