LOCUS       sequence-81007-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA 2 against rodent Rem2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Moore AR, Ghiretti AE, Paradis S
  TITLE     A loss-of-function analysis reveals that endogenous Rem2 promotes 
            functional glutamatergic synapse formation and restricts dendritic 
            complexity.
  JOURNAL   PLoS One. 2013 Aug 26;8(8):e74751. doi: 
            10.1371/journal.pone.0074751. eCollection 2013.
  PUBMED    23991227
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-26"; volume: "8(8):e74751. doi"; pages: " 
            10.1371/journal.pone.0074751. eCollection 2013"
COMMENT     Sequence Label: pSuper-Rem2-2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..66
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..66
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        86..300
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     221..240
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(373..389)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(374..390)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(418..436)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(457..473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(470..492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    481..497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(505..511,512..529,530..535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    550..571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(688..705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagaactag
       61 tggatccccc gggctgcagg aattcgaacg ctgacgtcat caacccgctc caaggaatcg
      121 cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc
      181 tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa
      241 tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac
      301 agatccccga actcagagga cacctatttc aagagaatag gtgtcctctg agttcttttt
      361 ggaaaagctt atcgataccg tcgacctcga gggggggccc ggtaccagct tttgttccct
      421 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
      481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
      541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
      601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
      661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      901 acgctcaagt cagaggtggc gaaacccgac aggactataa agatacca
//
