LOCUS       sequence-8101-ex        7990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1103
FEATURES             Location/Qualifiers
     source          1..7990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..3400
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(346..363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(346..362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(355..377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3634..3654
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3713..3799
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4488..5150)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      5151..5920
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(7203..7736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7925..7930,7931..7947,7948..7953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggattgg ctttactaag ctgatccggt ggatgacctt ttgaatgacc
       61 tttaatagat tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa
      121 aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac cggatctaga
      181 tatcgattaa ttaagtcgac atctagagtt taaactttaa ttaagcccgg gctgcagccc
      241 gggggatcca ctaggttaac gcatgcgggc ccggtaccag gcctagatag atagagagag
      301 agagagacta gtggaggaag aaaaaatgac catgattacg gattcactgg ccgtcgtttt
      361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      481 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
      541 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
      601 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc
      661 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
      721 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
      781 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
      841 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg
      901 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
      961 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
     1021 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
     1081 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
     1141 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
     1201 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
     1261 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
     1321 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
     1381 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
     1441 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
     1501 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
     1561 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
     1621 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
     1681 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
     1741 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
     1801 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
     1861 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
     1921 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
     1981 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
     2041 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
     2101 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
     2161 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
     2221 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
     2281 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
     2341 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
     2401 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
     2461 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
     2521 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
     2581 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
     2641 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
     2701 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
     2761 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
     2821 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
     2881 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
     2941 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
     3001 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
     3061 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
     3121 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
     3181 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
     3241 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
     3301 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
     3361 cggtcgctac cattaccagt tggtctggtg tcaaaaataa ctcgagtaca actataactc
     3421 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
     3481 tcgccacaac attgaagatg gatccgttca actagcagac cattatcaac aaaatactcc
     3541 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     3601 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
     3661 tgggattaca catggcatgg atgagctcta caaataatga attcccaggc atcaaataaa
     3721 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3781 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     3841 agggtggcgg gcaggacgcc cgccataaac tgccagggaa ttccaactga gcgccggtcg
     3901 ctaccattac caacttgtct ggtgtcaaaa ataataggat tttgttaaaa ttcgcgttaa
     3961 atttttgtta aatcagctca ttttttaacc aataggccga ctgcgatgag tggcagggcg
     4021 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat
     4081 aagtgataat aagcggatga atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt
     4141 tcagggcagg gtcgttaaat agccgcttat gtctattgct ggtttaccgg tttattgact
     4201 accggaagca gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc tcaggctctc
     4261 cccgtggagg taataattga cgatatgatc atttattctg cctcccagag cctgataaaa
     4321 acggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     4381 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgagg cggctacagc
     4441 cgatagtctg gaacagcgca cttacgggtt gctgcgcaac ccaagtgcta ccggcgcggc
     4501 agcgtgaccc gtgtcggcgg ctccaacggc tcgccatcgt ccagaaaaca cggctcatcg
     4561 ggcatcggca ggcgctgctg cccgcgccgt tcccattcct ccgtttcggt caaggctggc
     4621 aggtctggtt ccatgcccgg aatgccgggc tggctgggcg gctcctcgcc ggggccggtc
     4681 ggtagttgct gctcgcccgg atacagggtc gggatgcggc gcaggtcgcc atgccccaac
     4741 agcgattcgt cctggtcgtc gtgatcaacc accacggcgg cactgaacac cgacaggcgc
     4801 aactggtcgc ggggctggcc ccacgccacg cggtcattga ccacgtaggc cgacacggtg
     4861 ccggggccgt tgagcttcac gacggagatc cagcgctcgg ccaccaagtc cttgactgcg
     4921 tattggaccg tccgcaaaga acgtccgatg agcttggaaa gtgtcttctg gctgaccacc
     4981 acggcgttct ggtggcccat ctgcgccacg aggtgatgca gcagcattgc cgccgtgggt
     5041 ttcctcgcaa taagcccggc ccacgcctca tgcgctttgc gttccgtttg cacccagtga
     5101 ccgggcttgt tcttggcttg aatgccgatt tctctggact gcgtggccat gcttatctcc
     5161 atgcggtagg gtgccgcacg gttgcggcac catgcgcaat cagctgcaac ttttcggcag
     5221 cgcgacaaca attatgcgtt gcgtaaaagt ggcagtcaat tacagatttt ctttaaccta
     5281 cgcaatgagc tattgcgggg ggtgccgcaa tgagctgttg cgtacccccc ttttttaagt
     5341 tgttgatttt taagtctttc gcatttcgcc ctatatctag ttctttggtg cccaaagaag
     5401 ggcacccctg cggggttccc ccacgccttc ggcgcggctc cccctccggc aaaaagtggc
     5461 ccctccgggg cttgttgatc gactgcgcgg ccttcggcct tgcccaaggt ggcgctgccc
     5521 ccttggaacc cccgcactcg ccgccgtgag gctcgggggg caggcgggcg ggcttcgcct
     5581 tcgactgccc ccactcgcat aggcttgggt cgttccaggc gcgtcaaggc caagccgctg
     5641 cgcggtcgct gcgcgagcct tgacccgcct tccacttggt gtccaaccgg caagcgaagc
     5701 gcgcaggccg caggccggag gcttttcccc agagaaaatt aaaaaaattg atggggcaag
     5761 gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg aaggctgggt agccggtggg
     5821 caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc catcagcttg tccagcaggg
     5881 ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc tcgcggccat cgtccacata
     5941 tccacgggct ggcaagggag cgcagcgacc gcgcagggcg aagcccggag agcaagcccg
     6001 tagggcgccg cagccgccgt aggcggtcac gactttgcga agcaaagtct agtgagtata
     6061 ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc tgcccccgtc gagccggttg
     6121 gacaccaaaa gggaggggca ggcatggcgg catacgcgat catgcgatgc aagaagctgg
     6181 cgaaaatggg caacgtggcg gccagtctca agcacgccta ccgcgagcgc gagacgccca
     6241 acgctgacgc cagcaggacg ccagagaacg agcactgggc ggccagcagc accgatgaag
     6301 cgatgggccg actgcgcgag ttgctgccag agaagcggcg caaggacgct gtgttggcgg
     6361 tcgagtacgt catgacggcc agcccggaat ggtggaagtc ggccagccaa gaacagcagg
     6421 cggcgttctt cgagaaggcg cacaagtggc tggcggacaa gtacggggcg gatcgcatcg
     6481 tgacggccag catccaccgt gacgaaacca gcccgcacat gaccgcgttc gtggtgccgc
     6541 tgacgcagga cggcaggctg tcggccaagg agttcatcgg caacaaagcg cagatgaccc
     6601 gcgaccagac cacgtttgcg gccgctgtgg ccgatctagg gctgcaacgg ggcatcgagg
     6661 gcagcaaggc acgtcacacg cgcattcagg cgttctacga ggccctggag cggccaccag
     6721 tgggccacgt caccatcagc ccgcaagcgg tcgagccacg cgcctatgca ccgcagggat
     6781 tggccgaaaa gctgggaatc tcaaagcgcg ttgagacgcc ggaagccgtg gccgaccggc
     6841 tgacaaaagc ggttcggcag gggtatgagc ctgccctaca ggccgccgca ggagcgcgtg
     6901 agatgcgcaa gaaggccgat caagcccaag agacggcccg agaccttcgg gagcgcctga
     6961 agcccgttct ggacgccctg gggccgttga atcgggatat gcaggccaag gccgccgcga
     7021 tcatcaaggc cgtgggcgaa aagctgctga cggaacagcg ggaagtccag cgccagaaac
     7081 aggcccagcg ccagcaggaa cgcgggcgcg cacatttccc cgaaaagtgc cacctggcgg
     7141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     7201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     7261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     7321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     7381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     7441 aacctgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     7501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     7561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     7621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     7681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     7741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     7801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     7861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     7921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     7981 cggtgtgcgt
//
