LOCUS       sequence-81026-e        6845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 68bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL815N
FEATURES             Location/Qualifiers
     source          1..6845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..44)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..44)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(72..89)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(72..88)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(81..103)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    291..315
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1616..2245
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2679..3746
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3812..4006
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4242..4264)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4350..4490
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4405..4424
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4505..4522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4676..5264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4756..4775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5351..6145)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6570..6594
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6671..6845
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cgagctcggt acccggggat ccactagttc tagagtcgac ctgcaggcat
       61 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      121 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      181 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg
      241 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
      301 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
      361 gcgtgaaaag gtttatccgt tggtccattt gtatgtccat gccaaccaca gggttcccct
      421 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
      481 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
      541 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
      601 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
      661 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
      721 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
      781 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
      841 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
      901 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
      961 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     1021 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     1081 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     1141 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     1201 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     1261 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     1321 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     1381 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     1441 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     1501 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     1561 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     1621 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     1681 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     1741 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     1801 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     1861 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     1921 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     1981 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     2041 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     2101 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     2161 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     2221 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     2281 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     2341 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     2401 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     2461 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     2521 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     2581 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     2641 tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg
     2701 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     2761 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     2821 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     2881 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     2941 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     3001 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     3061 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     3121 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     3181 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     3241 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     3301 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     3361 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     3421 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     3481 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     3541 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     3601 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     3661 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     3721 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     3781 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa
     3841 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     3901 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     3961 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     4021 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     4081 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     4141 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     4201 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4261 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4321 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     4381 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4441 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     4501 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4561 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4621 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4741 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4801 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4861 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4981 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5041 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5101 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5161 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5221 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5281 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     5341 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     5401 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     5461 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     5521 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     5581 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     5641 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     5701 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     5761 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     5821 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     5881 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     5941 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     6001 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     6061 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     6121 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     6181 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     6241 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     6301 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     6361 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     6421 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     6481 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     6541 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     6601 acgcttagac aacttaataa cacattgcgg acgtttttat gtactgaatt aacgccgaat
     6661 taattcgggg gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     6721 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     6781 agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg
     6841 agaaa
//
