LOCUS       sequence-81031-e       10095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introducing ectopic integration at the defined fungal ILV2-locus, 
            based on SRR (Sulfonylurea Resistance Reconstitution)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Yang F, Naqvi NI
  TITLE     Sulfonylurea resistance reconstitution as a novel strategy for 
            ILV2-specific integration in Magnaporthe oryzae.
  JOURNAL   Fungal Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005.
  PUBMED    24769367
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2014 Apr 24. pii: S1087-1845(14)00059-0. doi: 
            10.1016/j.fgb.2014.04.005."
COMMENT     Sequence Label: pFGL889
FEATURES             Location/Qualifiers
     source          1..10095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2393..2409)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2393..2409)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3322..3339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3322..3338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3331..3353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3541..3565
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4866..5495
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5929..6996
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7062..7256
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7492..7514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7600..7740
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7655..7674
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7755..7772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7926..8514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8006..8025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8601..9395)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9820..9844
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9921..10095
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatc acgagcgagc gaacaaacag gcagagttac aaacgccatc actacgcctt
       61 gtcttttcgt ctctgttacg tgtcgccacc cccatgcgag ctacacgtct gcgcctgaag
      121 tcacaaggct tgcaatttca tgacgatgaa caacactgac acttctgtta cagagcacct
      181 cgaactaagc ccagcgccag cttcaatgct cgccgcgatc cccagcctct ggtcaaccct
      241 cgctcaggtg aggcagacga atcgtaagtt gcgccaacac cgctcctaca ccgccaagac
      301 ccataccgcc gtcgcatttg gacaagcaca agactgattt gaccgtggca tagattcatt
      361 ggcaagacgg gaggagagat tttccacgag atgatgctga ggcaaaacgt caagcatatc
      421 tgtaagcgtc taattctaca aattcctacc ctcgatttca accacatatt caaccacata
      481 ttctgacaca tgagtcacag tcggttaccc tggcggtgct atccttcccg tgttcgacgc
      541 gatctacaac tcgaagcaca tcgactttgt tctgcccaag catgagcaag gcgccggcca
      601 catggcagag ggctatgctc gcgcttcagg caaacccggc gttgttctcg tcacctccgg
      661 ccccggtgcc acaaatgtca tcactcccat ggccgacgct cttgccgacg gtacacctct
      721 ggttgtattc tcaggacagg ttgttacctc tgatattgga agcgacgcct tccaggaggc
      781 cgacgtcata ggcatctccc ggtcttgcac caagtggaac gtcatggtta agagcgctga
      841 cgagctcccg aggagaatta acgaggcctt tgagattgcc accagtgggc gacctgggcc
      901 tgtcttggtc gatctgccca aggacgtcac ggctagtgtg ctgaggaggg ctatccccac
      961 cgagacctcg attccctcta ttagcgcagc agcacgggct gtccaagagg caggccgaaa
     1021 gcagcttgag cactccatca aacgcgtagc cgatctcgtc aacattgcca agaagcccgt
     1081 catatatgcc ggccaaggtg tcattttgtc ggaaggcggc gttgaacttc tcaaggcgct
     1141 tgccgacaag gcctcgattc ctgtcaccac cactctgcat ggtctgggag cctttgacga
     1201 gctcgacgag aaggcactgc acatgcttgg tatgcacggt tcggcttatg ccaacatgtc
     1261 catgcaagag gccgatttga tcattgccct tggtggccgc ttcgatgacc gtgtcactgg
     1321 cagcatcccc aaatttgctc ctgccgccaa gctagctgct gctgaaggac gcggaggtat
     1381 tgtccacttc gagattatgc ccaagaacat caacaaggtc gtccaagcaa cagaggccat
     1441 tgagggcgac gttgcttcga acttgaagct gttgctcccc aagattgaac aacgatccat
     1501 gaccgatcgc aaggagtggt tcgaccagat caaggagtgg aaggagaagt ggcctctgtc
     1561 acattatgag agggccgagc gtagtggtct catcaagcct cagactctga tcgaggagct
     1621 gagcaacctg actgctgacc gcaaggacat gacctacatc acaaccggtg ttggccagca
     1681 ccaaatgtgg acagcacaac atttcaggtg gaggcaccca cggtccatga tcacctctgg
     1741 cggtttggga accatgggat atggtctgcc ggcagcgatt ggcgccaagg ttgctaggcc
     1801 agatgctttg gtcattgaca tcgacggcga cgcatcgttc aacatgactc tgacagagct
     1861 ttcgacggcg gcacagttca acattggcgt caaggtcatt gtcttgaaca acgaggagca
     1921 gggaatggtg acccaatggc agaacttgtt ctacgaggac cgctactcac atacacacca
     1981 gcgcaaccca gacttcatga agctcgccga tgcaatggac gttcaacatc gccgtgtttc
     2041 gaagcctgac gatgtcgttg atgctctgac gtggctgatc aacaccgacg gccccgctct
     2101 gcttgaggtg atgactgata agaaggttcc tgttctgccc atggtgcccg gaggtaacgg
     2161 cctgcacgag ttcatcacgt ttgatgccag taagtaatat atccaatgca ctcctgtggc
     2221 ccattttgag ccttgggtat gattctagtt caacttgcta accagtgcat caactcgtat
     2281 aggcaaggat aagcaacgga gagagctgat gcgcgcgagg acgaatggcc tgcacggtta
     2341 agttgataat gggaattgat tattgcacgg gaattcgagc tcggtacccg gggatccact
     2401 agttctagag tcgacctgca ggcatgctcc acagggacaa ataccctcca ggggtaattc
     2461 tattttggac aaaaagttgg aacttttgtg ataccatggt gttaagaatg tcttatggac
     2521 aaggggacga tacatttgag ctgcgggaat ggttagcggc ttgtgtaaat attgcatcat
     2581 tttttttttc tcttttactt ttttcttctt cttctggctt ggtattcgtg cgtttaattc
     2641 gtttcattct tctactttat tcgaagcaca ttccttgcgt catcagtatc attgatacaa
     2701 gtgatgtttc caactgaaca aatgttacaa cattggtccg ttccagcctt tgttcatgag
     2761 cagcaccggt atttgaacct ccaggttaga gtagcctggg aaatgcaacg agcattctgc
     2821 ctaatactag ctgccactcg aaggcattta tagttttgct gaggtttcaa tgacgcccct
     2881 ctgaagacaa agtagcacaa cggcattgac gagactacta ctggtggttg aactgacttt
     2941 tgcaccttgc tgctgctctt tggaagaggc ttgaccatcc actgcggaag aggtgcttgt
     3001 tgccttggga tttacttact tgtccgtcat cttggcggac tgggccgagc aaccaaccag
     3061 catcaacctt ggtcgcagaa gacattttga atcttggcaa agtgtgctat ctattttggg
     3121 cgtggaaatg gttcttgagt ttgttaggtg ggccctggga gggatgcgcg gtcaactcct
     3181 cacgactgca taaatctaat ggagatgaag gtgtcgttgg agaagttgca gattatatcg
     3241 gtgcatgaag catttgaaca gtcccaaatt ataccacttt catcgataca attctgtcag
     3301 tgactggttg tgaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3361 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     3481 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     3541 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     3601 atttaaaagg gcgtgaaaag gtttatccgt tggtccattt gtatgtccat gccaaccaca
     3661 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     3721 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
     3781 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     3841 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     3901 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     3961 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     4021 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     4081 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     4141 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     4201 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     4261 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     4321 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     4381 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     4441 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     4501 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     4561 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     4621 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg
     4681 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     4741 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     4801 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     4861 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     4921 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     4981 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     5041 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     5101 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     5161 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     5221 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     5281 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     5341 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     5401 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     5461 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     5521 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     5581 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     5641 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     5701 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
     5761 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     5821 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
     5881 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca
     5941 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     6001 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     6061 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     6121 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     6181 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6241 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6301 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6361 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6421 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     6481 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     6541 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     6601 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     6661 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     6721 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     6781 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     6841 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     6901 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     6961 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     7021 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     7081 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     7141 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     7201 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7261 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7321 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7381 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7441 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7501 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7561 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     7621 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7681 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7741 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7801 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7861 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7921 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7981 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8041 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8101 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8161 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8221 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8281 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8341 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8401 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8461 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8521 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8581 ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
     8641 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
     8701 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
     8761 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
     8821 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
     8881 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
     8941 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
     9001 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
     9061 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
     9121 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
     9181 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
     9241 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
     9301 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
     9361 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
     9421 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
     9481 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
     9541 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
     9601 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
     9661 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
     9721 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
     9781 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     9841 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttat gtactgaatt
     9901 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag
     9961 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
    10021 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
    10081 attattatgg agaaa
//
