LOCUS       sequence-81038-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant expression in bacteria of SpyLigase. The 
            Howarth lab has created two improved peptide ligation enzymes. See 
            depositor comments below!.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Fierer JO, Veggiani G, Howarth M
  TITLE     SpyLigase peptide-peptide ligation polymerizes affibodies to enhance
            magnetic cancer cell capture.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 17.
  PUBMED    24639550
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Mar 17."
COMMENT     Sequence Label: SpyLigase
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             421..438
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(491..509)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      505..552
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      589..947
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(676..695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     886..907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgtcgta ctaccatcac catcaccatc
       61 acgattacga cggtcagtcc ggtgacggca aagagttagc tggtgcaact atggagttgc
      121 gtgattcatc tggtaaaact attagtacat ggatttcaga tggacaagtg aaagatttct
      181 acctgtatcc aggaaaatat acatttgtcg aaaccgcagc accagacggt tatgaggtag
      241 caactgctat tacctttaca gttaatgagc aaggtcaggt tactgtaaat ggcaaagcaa
      301 ctaaaggtgg gagtggtggc agcggaggta gtggcgagga cagcgctacc catatttaaa
      361 tggttgatgc ttgaggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
      421 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      481 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      541 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      601 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      661 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      721 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      781 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      841 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      901 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttat
//
