LOCUS       sequence-81039-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant expression in bacteria of SUMO domain fused 
            to KTag, permitting covalent linkage to SpyTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Fierer JO, Veggiani G, Howarth M
  TITLE     SpyLigase peptide-peptide ligation polymerizes affibodies to enhance
            magnetic cancer cell capture.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 17.
  PUBMED    24639550
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Mar 17."
COMMENT     Sequence Label: SUMO-KTag
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             430..447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(500..518)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      514..561
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      598..956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(685..704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     895..916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tccctctaga ataattttgt ttaactttaa gaaggagata taccatgggt agcagccatc
       61 accatcatca tcacgggagc gatagcgaag tgaaccagga agcgaaaccg gaagttaaac
      121 cggaagtgaa accggaaacc catattaatc tgaaagttag cgacggcagc agcgaaatct
      181 tttttaaaat taaaaaaacc accccgctgc gtcgcctgat ggaagccttt gcgaaacgtc
      241 agggtaaaga aatggatagc ctgcgctttc tgtatgacgg catccgtatt caggccgatc
      301 agaccccgga agacctggat atggaagaca acgatattat tgaagcgcat cgcgaacaga
      361 tcggtggtgg cgctacccat attaaattct caaaacgtga tggttattaa gcttgcggcc
      421 gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa
      481 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      541 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
      601 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      661 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      721 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      781 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      841 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
      901 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataa
//
