LOCUS       sequence-81044-e        7143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length C-type lectin domain family 2 member C 
            ectodomain in mammalian cells. N-terminal His tag, biotinylation 
            sequence and rat Cd4d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: CD69-His-bio
FEATURES             Location/Qualifiers
     source          1..7143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1243..1298
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1297..1316)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3534..3552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3620..3724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3725..3793,3794..4585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3943..3962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4756..5344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5245..5264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5498..5515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5632..5653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5668..5673,5674..5691,5692..5698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5706..5722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5711..5733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5730..5746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5730..5746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6017..6396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6397..6600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6551..6571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6597..6621
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7102..7121
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcccggac agtacacctt cagcatgccc agcgacagcc acgtgtccag ctgcagcgag
      841 gactgggtcg gataccagcg gaagtgctac ttcatcagca ccgtgaagcg gagctggacc
      901 agcgcccaga acgcctgttc tgagcacggc gccaccctgg ccgtgatcga cagcgagaag
      961 gatatgaact tcctgaagag atacgccggc agagaggaac attgggtcgg actgaagaaa
     1021 gagcccggcc acccctggaa gtggtccaac ggcaaagagt tcaacaactg gttcaacgtg
     1081 accggcagcg ataagtgcgt gttcctgaag aacaccgagg tgtccagcat ggaatgcgag
     1141 aagaacctgt actggatctg caacaagccc tacaagtgag gcgcgcctga attcttctag
     1201 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     1261 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     1321 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     1381 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     1441 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     1501 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     1561 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     1621 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     1681 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     1741 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     1801 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     1861 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     1921 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     1981 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     2041 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     2101 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     2161 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     2221 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     2281 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     2341 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     2401 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     2461 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     2521 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     2581 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     2641 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     2701 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     2761 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     2821 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     2881 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     2941 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     3001 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     3061 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     3121 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     3181 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     3241 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     3301 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     3361 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     3421 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     3481 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     3541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3721 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3781 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3841 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3901 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3961 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4021 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4081 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4141 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4201 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4261 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4321 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4381 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4441 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4501 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4561 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4621 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4681 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4741 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4801 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4861 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4921 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4981 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5041 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5101 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5161 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5221 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5281 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5341 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5401 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5461 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5521 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5581 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5641 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5701 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5761 gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     5821 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
     5881 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     5941 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     6001 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     6061 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6241 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     6301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     6361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
     6421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     6481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     6541 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     6601 cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg
     6661 ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac
     6721 ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg
     6781 atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac
     6841 cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat
     6901 gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga
     6961 gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat
     7021 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact
     7081 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag
     7141 ttt
//
