LOCUS       sequence-81045-e        7287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length C-type lectin domain family 1 member B 
            ectodomain in mammalian cells. N-terminal His tag, biotinylation 
            sequence and rat Cd4d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: CLEC1B-His-bio
FEATURES             Location/Qualifiers
     source          1..7287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1387..1442
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1441..1460)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3678..3696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3764..3868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3869..3937,3938..4729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4087..4106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4900..5488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5389..5408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5642..5659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5776..5797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5812..5817,5818..5835,5836..5842)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5850..5866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5855..5877
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5874..5890
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5874..5890
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6161..6540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6541..6744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6695..6715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6741..6765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7246..7265
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcccggaa tttggagcgt gatgcagcgg aactacctgc agggcgagaa cgagaaccgg
      841 accggcaccc tgcagcagct ggccaagcgg ttctgccagt acgtggtcaa gcagagcgag
      901 ctgaagggca ccttcaaggg ccacaagtgc agcccctgcg acaccaactg gcggtactac
      961 ggcgacagct gctacggctt cttccggcac aacctgacct gggaggaaag caagcagtac
     1021 tgcaccgaca tgaacgccac cctgctgaag atcgacaacc ggaacatcgt cgagtacatc
     1081 aaggcccgga cacatctgat tagatgggtc ggactgagcc ggcagaaaag caacgaagtg
     1141 tggaagtggg aggacggcag cgtgatcagc gagaatatgt tcgagttcct cgaggacggc
     1201 aagggcaaca tgaactgcgc ctacttccac aacggcaaga tgcaccccac cttttgcgag
     1261 aacaagcact acctgatgtg cgagcggaag gccggcatga ccaaggtgga ccagctgccc
     1321 tgaggcgcgc ctgaattctt ctagacacgt gattaattaa ggatcccccg acctcgacct
     1381 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     1441 cactcggaag gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag
     1501 ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc
     1561 ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct
     1621 gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca
     1681 tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact
     1741 ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct
     1801 tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca
     1861 ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta
     1921 gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt
     1981 agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc
     2041 atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc
     2101 atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt
     2161 tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg
     2221 cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa
     2281 ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga
     2341 cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg
     2401 gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg
     2461 gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca
     2521 acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc
     2581 aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc
     2641 gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg
     2701 ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg
     2761 ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat
     2821 aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac
     2881 taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat
     2941 tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt
     3001 tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca
     3061 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg
     3121 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     3181 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     3241 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     3301 ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata
     3361 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag catatgctat
     3421 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     3481 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     3541 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     3601 cctaatctgt atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt
     3661 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3721 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4081 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4381 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     4441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5041 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     5701 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5761 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5821 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     5881 cagctatgac catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac
     5941 agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag
     6001 gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat
     6061 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     6121 tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt
     6181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6421 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6481 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6541 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     6601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6661 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     6721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
     6781 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
     6841 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
     6901 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
     6961 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
     7021 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
     7081 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
     7141 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
     7201 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
     7261 caggtgtcca ctcccaggtc caagttt
//
