LOCUS       sequence-81046-e        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains a C-terminal tandem affinity purification tag (relevant to 
            any system) within a construct designed for genome integration into 
            trypanosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Schimanski B, Nguyen TN, Gunzl A
  TITLE     Highly efficient tandem affinity purification of trypanosome protein
            complexes based on a novel epitope combination.
  JOURNAL   Eukaryot Cell. 2005 Nov;4(11):1942-50.
  PUBMED    16278461
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2005-11"; volume: "4(11)"; pages: "1942-50"
COMMENT     Sequence Label: pC-PTP-NEO
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             791..826
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             851..871
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccctaacag agcaggccac ccccaccgtt
       61 tgtcccccag tgccgccgac gtcgtgagcc aatagcttcg agtccggtcc atatacttca
      121 caagcgggca ctggagcttg ttgccgctga gcgaccgcct gtggtttcgt cgtcgtcaat
      181 gaatgcgtgg cccgttgcag cagcagagaa atctgaccct aagcgttcgg cctggttgga
      241 gcagatgaag tcggagatga atcgccgcaa tccgtataca gatttgaata tatgctcgga
      301 cccgcggtgc acagttgtga tgtcggggtt gcacgccaaa acggtggagg aggatatccg
      361 catcttcagc gagcagtttg gtcgtgtggt cagcactcgt ctaatttttg accgtaatgg
      421 tcacagccga cggtatggct ttgtgcagtt tggacgggag gcggatgtgc agagagcagt
      481 tgcaagtagt ggaaagaggc ggcttcacgg tcggagtgtt gtgatcgacg tggagcgagg
      541 gcggcaggaa cctgagtttc tgccgaaacg cattgcaaaa gctgtgaaat tgcaggggac
      601 gtcgatcaat tcccccgttt tgccgtctgg agacacgcca attggcaagc gccagcgcaa
      661 tgagaccgaa cggcaacaag atcgatcaag ggaggctcca actaagccgg agccatcagg
      721 aggtgaactg agacaccgtg atgatgtgga catccttctg gatgatatca tgagtattac
      781 gcggccgctg gaagatcagg tggatcctcg tcttattgat gggaaatatg atattccaac
      841 tactgctagc gagaatttgt attttcaggg tgagctcaaa accgcggctc ttgcgcaaca
      901 cgatgaagcc gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
      961 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     1021 cccaagccaa agcgctaacc t
//
