LOCUS       sequence-81049-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains a C-terminal tandem affinity purification tag (relevant to 
            any system) within a construct designed for genome integration into 
            trypanosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Schimanski B, Nguyen TN, Gunzl A
  TITLE     Highly efficient tandem affinity purification of trypanosome protein
            complexes based on a novel epitope combination.
  JOURNAL   Eukaryot Cell. 2005 Nov;4(11):1942-50.
  PUBMED    16278461
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2005-11"; volume: "4(11)"; pages: "1942-50"
COMMENT     Sequence Label: pC-PTP-NEO
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(903..922)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc tcgataaata aatagaagtg ctttgttgtt
       61 gttgttagtg gtgctagtac ctactgttgc taatataaac ggatgaatag tcctttgctt
      121 aaactagtgt ttgaagatct gtcagaaatc agcaccgcga gggggtgagg agaggagaac
      181 atagagtcaa cattaggcaa tacttacgcc accaccactt acgaagtgag aagtgcgtgt
      241 aggcgcactc cacacagcgg aagaggcaaa caatgggacc cataaaggtg gaggatcaag
      301 attgtgtaca tctaacgaga agaaggcgtg ttgatgagtt gtactgttga tctcactagc
      361 tcatcttaca cgtgacggtt catacatagt cacgtaagga cagacaggga ccccaataca
      421 tcccacatca tggtacgcgt gtccctcata tacccttccc acctcctcta ctatccgtgg
      481 ctctctgaat agagtggcgc gcggatgtac acaaacgtgg aagaaaaaaa aaattaaaga
      541 aaacaacaac aaaacataaa cacaaaagaa caaaatgaca gaaggacgaa ggagaaaggg
      601 gacgagaaac aaacatcttc attacctact gctaatgacg aacgggaaat gccccgtccg
      661 cgtctagtat tgctcctcct cgtcgaactc gccctcctcc tcaatcgtgg catcctggta
      721 ctgctggtac tcagacacga gatcgttcat gttggactct gcctccgtga attttcgaag
      781 ttaacggatc ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
      841 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
      901 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
      961 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatc
//
