LOCUS       sequence-8105-ex        5311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1144
FEATURES             Location/Qualifiers
     source          1..5311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             326..1003
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             326..1003
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(456..474)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     724..743
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     918..941
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      1034..1120
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1809..2471)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      2472..3241
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(4524..5057)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5246..5251,5252..5268,5269..5274))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccatgggcaa atgggattgg ctttactaag ctgatccggt ggatgacctt ttgaatgacc
       61 tttaatagat tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa
      121 aattttttca caaaacggtt tacaagcata aagcttgctc aatcaatcac cggatctaga
      181 tatcgattaa ttaagtcgac atctagagtt taaactttaa ttaagcccgg gctgcagccc
      241 gggggatcca ctaggttaac gcatgcgggc ccggtaccag gcctagatag atagagagag
      301 agagagacta gtggaggaag aaaaaatggc ctcctccgag gacgtcatca aggagttcat
      361 gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      421 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
      481 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc cagtacggct ccaaggccta
      541 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      601 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      661 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      721 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc ggatgtaccc
      781 cgaggacggc gccctgaagg gcgagatcaa gatgaggctg aagctgaagg acggcggcca
      841 ctacgacgcc gaggtcaaga ccacctacat ggccaagaag cccgtgcagc tgcccggcgc
      901 ctacaagacc gacatcaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
      961 acagtacgag cgcgccgagg gccgccactc caccggcgcc taagagctct acaaataatg
     1021 aattcccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1081 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     1141 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggga
     1201 attccaactg agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataatagga
     1261 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1321 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     1381 cgcctggtgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc
     1441 aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc
     1501 tggtttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga
     1561 ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat catttattct
     1621 gcctcccaga gcctgataaa aacggtgaat ccgttagcga ggtgccgccg gcttccattc
     1681 aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata
     1741 gggcggcgag gcggctacag ccgatagtct ggaacagcgc acttacgggt tgctgcgcaa
     1801 cccaagtgct accggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg ctcgccatcg
     1861 tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg ttcccattcc
     1921 tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg ctggctgggc
     1981 ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt cgggatgcgg
     2041 cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac caccacggcg
     2101 gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac gcggtcattg
     2161 accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat ccagcgctcg
     2221 gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat gagcttggaa
     2281 agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac gaggtgatgc
     2341 agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc atgcgctttg
     2401 cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat ttctctggac
     2461 tgcgtggcca tgcttatctc catgcggtag ggtgccgcac ggttgcggca ccatgcgcaa
     2521 tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag tggcagtcaa
     2581 ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca atgagctgtt
     2641 gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc cctatatcta
     2701 gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt cggcgcggct
     2761 ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg gccttcggcc
     2821 ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga ggctcggggg
     2881 gcaggcgggc gggcttcgcc ttcgactgcc cccactcgca taggcttggg tcgttccagg
     2941 cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc ttgacccgcc ttccacttgg
     3001 tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga ggcttttccc cagagaaaat
     3061 taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg gagccggtgg gtatgtggtc
     3121 gaaggctggg tagccggtgg gcaatccctg tggtcaagct cgtgggcagg cgcagcctgt
     3181 ccatcagctt gtccagcagg gttgtccacg ggccgagcga agcgagccag ccggtggccg
     3241 ctcgcggcca tcgtccacat atccacgggc tggcaaggga gcgcagcgac cgcgcagggc
     3301 gaagcccgga gagcaagccc gtagggcgcc gcagccgccg taggcggtca cgactttgcg
     3361 aagcaaagtc tagtgagtat actcaagcat tgagtggccc gccggaggca ccgccttgcg
     3421 ctgcccccgt cgagccggtt ggacaccaaa agggaggggc aggcatggcg gcatacgcga
     3481 tcatgcgatg caagaagctg gcgaaaatgg gcaacgtggc ggccagtctc aagcacgcct
     3541 accgcgagcg cgagacgccc aacgctgacg ccagcaggac gccagagaac gagcactggg
     3601 cggccagcag caccgatgaa gcgatgggcc gactgcgcga gttgctgcca gagaagcggc
     3661 gcaaggacgc tgtgttggcg gtcgagtacg tcatgacggc cagcccggaa tggtggaagt
     3721 cggccagcca agaacagcag gcggcgttct tcgagaaggc gcacaagtgg ctggcggaca
     3781 agtacggggc ggatcgcatc gtgacggcca gcatccaccg tgacgaaacc agcccgcaca
     3841 tgaccgcgtt cgtggtgccg ctgacgcagg acggcaggct gtcggccaag gagttcatcg
     3901 gcaacaaagc gcagatgacc cgcgaccaga ccacgtttgc ggccgctgtg gccgatctag
     3961 ggctgcaacg gggcatcgag ggcagcaagg cacgtcacac gcgcattcag gcgttctacg
     4021 aggccctgga gcggccacca gtgggccacg tcaccatcag cccgcaagcg gtcgagccac
     4081 gcgcctatgc accgcaggga ttggccgaaa agctgggaat ctcaaagcgc gttgagacgc
     4141 cggaagccgt ggccgaccgg ctgacaaaag cggttcggca ggggtatgag cctgccctac
     4201 aggccgccgc aggagcgcgt gagatgcgca agaaggccga tcaagcccaa gagacggccc
     4261 gagaccttcg ggagcgcctg aagcccgttc tggacgccct ggggccgttg aatcgggata
     4321 tgcaggccaa ggccgccgcg atcatcaagg ccgtgggcga aaagctgctg acggaacagc
     4381 gggaagtcca gcgccagaaa caggcccagc gccagcagga acgcgggcgc gcacatttcc
     4441 ccgaaaagtg ccacctggcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     4501 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     4561 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     4621 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     4681 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     4741 atctcactac gcggctgctc aaacctgggc agaacgtaag ccgcgagagc gccaacaacc
     4801 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     4861 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     4921 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     4981 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     5041 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     5101 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     5161 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     5221 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     5281 tcgtgccttc atccgtttcc acggtgtgcg t
//
