LOCUS       sequence-81050-e        8250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Metastasis adhesion protein ectodomain in 
            mammalian cells. N-terminal His tag, biotinylation sequence and rat 
            Cd4d3+4 tag. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: MTDH-His-bio
FEATURES             Location/Qualifiers
     source          1..8250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2350..2405
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2404..2423)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4641..4659)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4727..4831
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4832..4900,4901..5692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5050..5069)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5863..6451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6352..6371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6605..6622
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6739..6760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6775..6780,6781..6798,6799..6805)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6813..6829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6818..6840
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6837..6853
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6837..6853
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7124..7503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7504..7707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7658..7678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7704..7728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     8209..8228
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcctagcc cccccagaaa gagagaagag gccgctgccg tgcccgctgc cgctcctgat
      841 gatctggccc tgctgaagaa cctgcgctcc gaggaacaga agaagaagaa ccggaagaag
      901 ctgagcgaga agcccaagcc caacggccgg accgtggaag tggccgaagg cgaggctgtg
      961 cggacccctc agagcgtgac cgccaagcag ccccccgaga tcgacaagaa gaacgagaag
     1021 tctaagaaga acaagaaaaa gtccaagagc gacgccaagg ccgtgcagaa cagcagccgg
     1081 cacgacggca aagaggtgga cgagggcgcc tgggagacaa agatcagcca cagagagaag
     1141 cggcagcagc ggaagagaga caaggtcctg accgacagcg gcagcctgga cagcaccatc
     1201 cccggcatcg agaacaccat caccgtgaca acagagcagc tgaccaccgc cagcttcccc
     1261 gtgggcagca agaaaaacaa gggcgacagc catctgaacg tccaggtctc caacttcaag
     1321 agcggcaagg gcgatagcac cctccaggtg tcaagcggcc tgaacgagaa cctgaccgtg
     1381 aacggcggag gctggaatga gaagtccgtg aagctgagca gccagatctc tgccggcgaa
     1441 gagaagtgga acagcgtgtc ccctgccagc gccggcaaga gaaagaccga gcctagcgcc
     1501 tggtcccagg ataccggcga cgccaacacc aacggcaagg actggggccg gtcttggagc
     1561 gaccggtcca tcttcagcgg catcggcagc accgccgagc ctgtgtctca gagcaccacc
     1621 agcgactacc agtgggacgt gtcccggaac cagccctaca tcgacgacga gtggtccggc
     1681 ctgaatggcc tgagcagcgc cgaccccaac agcgactgga acgcccctgc cgaggaatgg
     1741 ggcaactggg tggacgaaga gagagccagc ctgctgaagt cccaggaacc catccccgac
     1801 gaccagaagg tgtccgacga cgacaaagag aagggcgaag gagccctgcc cacaggcaag
     1861 tccaagaaga aaaaaaagaa aaagaagaag cagggcgagg acaacagcac cgcccaggac
     1921 accgaggaac tggaaaaaga gatccgcgag gacctgcccg tgaataccag caagacccgg
     1981 cccaagcagg aaaaggcctt cagcctgaaa accatcagca ccagcgaccc agccgaggtg
     2041 ctggtcaaga acagccagcc catcaagacc ctgccccctg ccaccagcac cgagccatct
     2101 gtgatcctga gcaagagcga cagcgacaag agcagcagcc aggtgccccc catcctgcag
     2161 gaaaccgata agagcaagag caacaccaag cagaactccg tgcctcctag ccagaccaag
     2221 agcgagacaa gctgggagag ccccaagcag atcaagaaga agaaaaaggc caggcgggaa
     2281 acctgaggcg cgcctgaatt cttctagaca cgtgattaat taaggatccc ccgacctcga
     2341 cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     2401 tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc
     2461 tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
     2521 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
     2581 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
     2641 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
     2701 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
     2761 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
     2821 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
     2881 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
     2941 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
     3001 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
     3061 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
     3121 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
     3181 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
     3241 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
     3301 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
     3361 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
     3421 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
     3481 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
     3541 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
     3601 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
     3661 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
     3721 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
     3781 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
     3841 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
     3901 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
     3961 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
     4021 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     4081 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4141 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4201 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     4261 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
     4321 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
     4381 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4441 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4501 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4561 tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga
     4621 attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4681 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4741 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4801 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4861 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4921 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4981 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5041 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5101 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5161 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5221 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5281 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5341 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5401 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5461 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5521 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5581 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5641 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5701 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5761 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5821 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5881 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5941 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6001 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6061 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6121 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6181 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6241 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6301 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6361 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6421 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6481 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6541 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6601 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6661 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6721 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6781 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6841 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt
     6901 gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg
     6961 taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     7021 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     7081 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
     7141 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7201 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7261 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7321 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7381 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7441 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7501 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga
     7561 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     7621 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
     7681 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc
     7741 cgcatcgctg tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg
     7801 tctttccagt actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag
     7861 ggacctgagc gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca
     7921 gtcacagtcg caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt
     7981 gtttctggcg gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat
     8041 ggtcgaggtg aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa
     8101 aagcgggcat tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     8161 tcacctggcc cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct
     8221 ccacaggtgt ccactcccag gtccaagttt
//
