LOCUS       sequence-81052-e        6999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Neuron-specific protein family member 1 
            ectodomain in mammalian cells. N-terminal His tag, biotinylation 
            sequence and rat Cd4d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: PR594-His-bio
FEATURES             Location/Qualifiers
     source          1..6999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1099..1154
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1153..1172)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3390..3408)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3476..3580
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3581..3649,3650..4441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3799..3818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4612..5200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5101..5120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5354..5371
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5488..5509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5524..5529,5530..5547,5548..5554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5562..5578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5567..5589
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5586..5602
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5586..5602
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5873..6252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6253..6456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6407..6427
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6453..6477
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6958..6977
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcctaagg tgtacaaata cgaccgggcc tgccccgacg gcttcgtgct gaagaacacc
      841 cagtgcatcc ccgagggcct ggaaagctac tacgccgagc aggacagcag cgccagagaa
      901 aagttctaca ccgtgatcaa ccactacaac ctggccaagc agagcatcac ccgcagcgtg
      961 tccccctgga tgagcgtgct gagcgaggaa aagctgagcg agcaggaaac cgaggccgcc
     1021 gagaagtccg cctgaggcgc gcctgaattc ttctagacac gtgattaatt aaggatcccc
     1081 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1141 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
     1201 ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     1261 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     1321 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     1381 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     1441 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     1501 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     1561 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     1621 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     1681 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     1741 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     1801 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     1861 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     1921 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     1981 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     2041 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     2101 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     2161 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     2221 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     2281 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     2341 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     2401 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     2461 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     2521 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     2581 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     2641 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     2701 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     2761 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     2821 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2881 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2941 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     3001 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     3061 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     3121 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     3181 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     3241 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     3301 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca
     3361 aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3421 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3481 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3541 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3601 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3661 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3721 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3781 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3841 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3901 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3961 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4021 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4081 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     4141 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4201 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4261 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4321 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4381 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4441 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4501 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4741 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5221 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5281 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5341 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5401 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5461 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5521 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5581 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt
     5641 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     5701 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     5761 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     5821 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     5881 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5941 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6001 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6061 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6121 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6181 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     6241 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     6301 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6361 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     6421 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcctc
     6481 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
     6541 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
     6601 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
     6661 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
     6721 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
     6781 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     6841 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     6901 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     6961 cctttctctc cacaggtgtc cactcccagg tccaagttt
//
