LOCUS       sequence-81054-e        7452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length Delta-sarcoglycan ectodomain in mammalian 
            cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 
            tag. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: SGCD-His-bio
FEATURES             Location/Qualifiers
     source          1..7452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1552..1607
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1606..1625)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3843..3861)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3929..4033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4034..4102,4103..4894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4252..4271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5065..5653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5554..5573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5807..5824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5941..5962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5977..5982,5983..6000,6001..6007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6015..6031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6020..6042
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6039..6055
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6039..6055
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6326..6705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6706..6909
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6860..6880
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6906..6930
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7411..7430
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcctaaag tgatgaactt caccatcgac ggcatgggca acctgcggat caccgagaag
      841 ggcctgaagc tggaaggcga cagcgagttc ctgcagcccc tgtacgccaa agagatccag
      901 agcagacccg gcaacgccct gtacttcaag agcgcccgga acgtgaccgt gaacatcctg
      961 aacgaccaga ccaaggtgct gacccagctg atcaccggcc ccaaggccgt ggaagcctac
     1021 ggcaagaaat tcgaagtgaa aaccgtgtcc ggcaagctgc tgttcagcgc cgacaacaac
     1081 gaggtggtcg tgggcgccga gcggctgaga gtgctgggag ctgagggcac cgtgttcccc
     1141 aagagcatcg agacacccaa cgtgcgggcc gaccccttca aagagctgcg gctggaaagc
     1201 cccacccggt ccctggtcat ggaagctcct aagggcgtcg aaatcaacgc cgaggccggc
     1261 aacatggaag ccacctgtcg gaccgagctg agactggaaa gcaaggacgg cgagatcaag
     1321 ctggacgccg ccaagatccg gctgcccaga ctgcctcacg gcagctacac ccccaccggc
     1381 acccggcaga aggtgttcga gatctgcgtg tgcgccaacg gccggctgtt cctgagtcag
     1441 gctggcgccg gaagcacctg tcagatcaac accagcgtgt gcctgtgagg cgcgcctgaa
     1501 ttcttctaga cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga
     1561 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     1621 atgggagggc aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc
     1681 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     1741 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     1801 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     1861 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     1921 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     1981 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     2041 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     2101 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     2161 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     2221 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     2281 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     2341 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     2401 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     2461 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     2521 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     2581 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     2641 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     2701 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     2761 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     2821 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     2881 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     2941 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     3001 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     3061 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     3121 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     3181 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     3241 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     3301 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     3361 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3421 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     3481 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     3541 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     3601 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     3661 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     3721 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     3781 ggtagcatat gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa
     3841 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3901 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3961 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4021 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4081 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4141 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4201 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4261 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     4321 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4381 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4441 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4501 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4561 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4621 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4681 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4741 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4801 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4861 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4921 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5761 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5821 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5881 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5941 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6001 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6061 ttacgccaag ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag
     6121 atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat
     6181 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     6241 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     6301 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     6361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     6601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     6661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6721 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6781 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6841 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6901 agcagagctc gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag
     6961 ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg
     7021 atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc
     7081 atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag
     7141 gctgagcacc gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct
     7201 gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg
     7261 caggcttgag atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg
     7321 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg
     7381 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
     7441 aggtccaagt tt
//
