LOCUS       sequence-81056-e        7140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length TPA-induced transmembrane protein ectodomain 
            in mammalian cells. N-terminal His tag, biotinylation sequence and 
            rat Cd4d3+4 tag. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts MF, Wright 
            GJ
  TITLE     A human platelet receptor protein microarray identifies 
            FcepsilonR1alpha as an activating PEAR1 ligand.
  JOURNAL   Mol Cell Proteomics. 2015 Feb 23. pii: mcp.M114.046946.
  PUBMED    25713122
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2015 Feb 23. pii: mcp.M114.046946."
COMMENT     Sequence Label: TTMP-His-bio
FEATURES             Location/Qualifiers
     source          1..7140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1240..1295
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1294..1313)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3531..3549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3617..3721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3722..3790,3791..4582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3940..3959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4753..5341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5242..5261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5495..5512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5629..5650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5665..5670,5671..5688,5689..5695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5703..5719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5708..5730
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5727..5743
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5727..5743
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6014..6393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6394..6597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6548..6568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6594..6618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     7099..7118
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg agtttcagac ccaggtactc atgtccctgc tgctctgcat gtctggtctg
       61 aattccggcc accaccatca ccaccatagc cccgacagga acctgcctcc tctggcccct
      121 ctgggcccca acagcggcag cccagacctg catcatattc tggatgcaca gaaaatggtg
      181 tggaatcatc gtgacagaaa cctgcctcca ctagcacctc tgtcgacctc catcacggcc
      241 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
      301 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
      361 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
      421 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
      481 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
      541 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
      601 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
      661 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
      721 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattcgcgg
      781 ccgcctgccg tgacatacgt ggacgaggac gagaacgaga tcctggaact gagcagcaac
      841 aagaccttct tcatcatgct gaagatcccc gaggaatgcg tggccgagga agaactgccc
      901 catctgctga ccgagcggct gaccgacgtg tacagcacca gccccagcct gagccggtac
      961 ttcaccagcg tggaaatcgt ggacttcagc ggcgagaacg ccaccgtgac ctacgacctg
     1021 cagttcggcg tgcccagcga cgacgagaac ttcatgaagt acatgatgag cgaggaactg
     1081 gtgctgggca tcctgctgca ggacttccgg gaccagaaca tccccggctg cgagagcctg
     1141 ggcctggatc ctaccagcct gctgctgtac gagtgaggcg cgcctgaatt cttctagaca
     1201 cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     1261 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     1321 atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg
     1381 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     1441 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     1501 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     1561 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     1621 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     1681 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     1741 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     1801 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     1861 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     1921 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     1981 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     2041 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     2101 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     2161 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     2221 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     2281 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     2341 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     2401 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     2461 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     2521 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     2581 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     2641 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     2701 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     2761 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     2821 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     2881 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     2941 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     3001 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     3061 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     3121 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     3181 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     3241 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     3301 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     3361 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     3421 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     3481 tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat
     3541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     3721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     3781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     3841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3901 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3961 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4021 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4081 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4141 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4261 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4561 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4621 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4861 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5401 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5461 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5521 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5581 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     5641 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     5701 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     5761 ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     5821 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     5881 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     5941 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     6001 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     6061 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6241 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     6301 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     6361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     6421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     6481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     6541 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     6601 ttagtgaacc gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg
     6661 ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt actcttggat cggaaacccg
     6721 tcggcctccg aacggtactc cgccaccgag ggacctgagc gagtccgcat cgaccggatc
     6781 ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc tgagcaccgt
     6841 ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg gaggtgctgc tgatgatgta
     6901 attaaagtag gcggtcttga gacggcggat ggtcgaggtg aggtgtggca ggcttgagat
     6961 ccagctgttg gggtgagtac tccctctcaa aagcgggcat tacttctgcg ctaagattgt
     7021 cagtttccaa aaacgaggag gatttgatat tcacctggcc cgatctggcc atacacttga
     7081 gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag gtccaagttt
//
