LOCUS       sequence-8106-ex       13103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1064
FEATURES             Location/Qualifiers
     source          1..13103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(216..244)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     839..859
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             909..2720
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             complement(2768..3916)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      4584..5294
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(5781..6431)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             6537..7736
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(8260..9051,9052..9120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI
                     ELDLNNGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATSLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGISAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            9402..9510
                     /note="incP origin of transfer"
     protein_bind    9698..9719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9734..9739,9740..9757,9758..9764)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9772..9788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9777..9799
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9796..9812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9796..9812
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccatggatcc aagcttgctc aatcaatcac cggatctaga tatcgattaa ttaagtcgac
       61 atctagagtt taaactttaa ttaagcccgg gctgcagccc gggggatcca ctaggttaac
      121 gcatgcgggc ccggtaccag gcctagatag atagagagag agagagacta gtggaggaag
      181 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      241 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      301 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      361 cacttgtcac tactttctct tatggtgttc aatgcttttc ccgttatccg gatcatatga
      421 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat
      481 ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
      541 ttgttaatcg tatcgagtta aaaggtattg attttaaaga agatggaaac attctcggac
      601 acaaactcga gtacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga
      661 atggaatcaa agctaacttc aaaattcgcc acaacattga agatggatcc gttcaactag
      721 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      781 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagcgt gaccacatgg
      841 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctagactg
      901 aggtaatcat ggtccgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc
      961 tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg
     1021 cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg
     1081 cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag
     1141 gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt
     1201 gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg
     1261 tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac
     1321 tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc
     1381 agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca
     1441 ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta
     1501 accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg
     1561 atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga
     1621 atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa
     1681 gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg
     1741 gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag
     1801 atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa
     1861 tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc
     1921 tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta
     1981 acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag
     2041 aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc
     2101 gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc
     2161 cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga
     2221 cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg
     2281 atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt
     2341 tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc
     2401 agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca
     2461 ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg
     2521 atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc
     2581 aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga
     2641 agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc
     2701 agcagggagg caaacaatga ctgcaggtgg ctgctgaacc cccagccgga actgacccca
     2761 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     2821 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     2881 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     2941 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     3001 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     3061 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     3121 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     3181 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     3241 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     3301 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     3361 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     3421 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     3481 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     3541 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     3601 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     3661 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     3721 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
     3781 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     3841 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     3901 caaacgtccg attcattcac cctccttgcg ggattgcccc ggaattcccc ggatcgatcc
     3961 gtcgatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     4021 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     4081 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     4141 ttctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     4201 cagcaccgtt tttgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     4261 cctgagtgct tgcggcagcg tgaagctgac ggatcgatcc ggggaaatta attcaccccc
     4321 gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa aaattgccgg
     4381 ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc cgccacccag
     4441 gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca cctgcggcac
     4501 gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg ccccgatccc
     4561 ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     4621 gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg
     4681 gggcaccccc cttcggcgtg cgcggtcacg cgccagggcg cagccctggt taaaaacaag
     4741 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
     4801 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
     4861 gtgcgcccct catctgtcat cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
     4921 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
     4981 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
     5041 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
     5101 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
     5161 gtggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg
     5221 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag
     5281 cgtcggaaag gcgctggaag ccccgtagcg acgcggagag gggcgagaca agccaagggc
     5341 gcaggctcga tgcgcagcac gacatagccg gttctcgcaa ggacgagaat ttccctgcgg
     5401 tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat tcgcgagagc cttgagtcca
     5461 cgctagatcc gtcaattcga tctatctcat ctgcgcaagg cagaacgtga agacggccgc
     5521 cctggacctc gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc
     5581 ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg
     5641 tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct
     5701 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg
     5761 cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca
     5821 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     5881 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     5941 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     6001 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     6061 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     6121 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     6181 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     6241 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     6301 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     6361 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     6421 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     6481 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     6541 aacccaacat acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     6601 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     6661 ccgcccacta tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg
     6721 tgctgggcgc gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg
     6781 gcgccactgt cgactacgcc atcatggcga cagcgccttt cctttgggtt ctctatatcg
     6841 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     6901 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     6961 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     7021 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     7081 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     7141 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     7201 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     7261 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     7321 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     7381 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     7441 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     7501 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     7561 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     7621 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     7681 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     7741 ggaaacgata gggaattgac ggatctatct catctgcgca aggcagaacg tgaagacggc
     7801 cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga cccgcgccac
     7861 ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg
     7921 ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg
     7981 tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg
     8041 ttgcgctccg gcagcggtcc tgatcccccg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcac tgataccgcg
     8401 agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gatgttgcca ttgctgcagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgggtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     8881 ggataatacc gcaccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgtt gttgagatcc agctcgatgt aacccacgcg
     9001 tgcacccaac ttatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct gggtcgatcg acggatcttt tccgctgcat aaccctgctt cggggtcatt
     9301 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     9361 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     9421 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     9481 aacgggaatc ctgctctgcg aggctggccg ataagctcta agaaaccatt attatcatga
     9541 cattaaccta taaaaataag cgtatcacga ggccctttcg tcttcaagaa ttaattccaa
     9601 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9661 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9721 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9781 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg atcccccgat
     9841 gtgtagcagt ggcggaccat ataggcagat cagaaggcgc ggttctccta catgttcgcc
     9901 ggtgaacgcg ttgaggaagc cgggcagtgc ctcggcaaaa tccttgcgtg tagacaagac
     9961 atctgcgtag cagttgtcct caacaacgat gtcgaaatcc aaatcggagt gctcatcgag
    10021 tcctccgtga acgtaagagc cgccgatcag aagagcgcgg aagcgaacat cggaagcgac
    10081 cgcatcgcgg atgcggttca agaaagttgc atgagcttgt ggaagtgtgc tgagcataaa
    10141 tgattctcct agctgttctt tgggtaagta cgccatcagg acgttgtgag tggcgcgatt
    10201 tttagcggct gaaatcagcc cttgagcctg tcggcaagtc gcgtcatgag gtccatgcgc
    10261 tcatgcagga tcgccacgac caacgcgggt tcgcccgcac gcggcaggca aaaaacgtag
    10321 tggtgttcgc agcgggccat ccgcagcgcg ggaaagagtt cgctcatgtc cttaaacggg
    10381 ccttcgccgg cggcaagcct ggctatgccc tgttccagct tagcgatata gcggcgcacc
    10441 tgcgccgcgc cccactcccg gcgcgtgtag cggatgatgc cgcgtagatc ggcttcggcc
    10501 tcagccgtga ggatgtaggc cgtcaagcgc gatccccgct gagttcttca tcaagaattt
    10561 cgccgacgct cttggtggac accttgccgg caagcccatc gttgatgcgg ttccccagca
    10621 tggttttcag ttcctgccat gcctgatcgg catcagcgtc accggggaac agacgttcga
    10681 gggcgtattg cttaatggtc ttgccctgca aggcggccag ggctttcagg ctctggtgct
    10741 gctggtccgt catgtcgatt gtcaggcggc tcattggata acctccataa aatacacgta
    10801 accacattag cacatatgtg ggcgtgaggc tacagcgcga ggcgcattaa ggtcgggaaa
    10861 atgcgctagg cgcatttaaa ttgcgtattg ctgtaatgcg ccatgccggc tagactaggc
    10921 ccaaatgggt atacccaatt tgaccaaggg ggacgcgatg agggcggcca agcactaccg
    10981 acaacttcta tccatcgact tcaacatcga ggcgctggcc ttcgtgcctg gacccgacgg
    11041 cacacgcggc cggcgcatcc acgtcctggg gcgcgaggtc cgcgaccggc ccggcctggt
    11101 cgagtacctt tcgccggcgt tcggctcgcg ggtggcgctg gacggctact gcaaggccaa
    11161 tttcgatgca gtgctgcacc tggcgtaccc cgatcatcag caatggggcc acgcatgaag
    11221 cgccgaagct acgccatgct gcgcgccgct gccgcgctgg ccgtcctggt cgttgcctcg
    11281 ccggcatggg ccgagctgcg cggcgaggtc gtgcgcatca tcgacggcga caccatcgac
    11341 gtgctggtag acaagcagcc ggtgcgcgtg cgcctggtgg acattgacgc gccggaaaag
    11401 cggcaagcct tcggcgaacg tgcgcgccag gcgctggccg gcatggtgtt ccgccggcac
    11461 gtcctggtcg acgagaagga caccgaccgt tacggccgca cgctgggcac cgtgtgggtc
    11521 aacatggagc tggccagccg gccgccgcag ccgcgcaacg tcaacgccgc gatggttcac
    11581 cagggcatgg cgtgggccta tcgcttccac ggccgcgcgg ccgaccctga aatgctgcgg
    11641 ctcgaacagg aggcgcgagg caagcgcgtc ggcctctggt ccgatccgca cgccgtcgag
    11701 ccgtggaaat ggcgacgcga gagcaacaac cggagggacg aaggttgaag gtcgcccgca
    11761 tctacctgcg cgccagtacg gacgagcaga atcttgaacg ccaggagagc cttgtagcgg
    11821 ccacgcgggc cgccgggtac tacgtcgccg gcatctaccg cgagaaggcg tccggcgcac
    11881 gcgccgaccg gcccgagctg ctgcgcatga tcgcggacct gcaacctggt gaagtcgtcg
    11941 ttgcggagaa gatcgaccgc atcagccgct tgccgttggc cgaggccgag cgcctggttg
    12001 cgtcgatccg ggccaaaggg gccaagctgg ccgtgcctgg cgtggtggac ctgtcggagc
    12061 tggccgccga ggcgaacgga gtggcgaaaa tcgttctgga atccgtccag gacatgcttt
    12121 tgaagctcgc cttgcagatg gcccgcgacg actacgagga tcggcgcgag cgtcaacgtc
    12181 agggtgtcca gttggcgaag gccgccggcc gctacaccgg ccgcaaacgt gacgccggca
    12241 tgcacgaccg catcatcacg cttcgctccg gcggatcgag cattgccaag acggccaagc
    12301 tggtcggatg cagcccgagc caggtcaaac gagtgtgggc ggcctggaac gcgcagcagc
    12361 aaaaataaag ccgggcagtg cccggctttt ctcacctttt cgcgtcccgc agggccgctg
    12421 cgagcgccct acctagatcc tcgctttccc cctcggtgta gtccggccag ggcacgaagg
    12481 gcgcggatgc gaacctgttg agcaggtacg ccttcgggca gcggtagacc accggcgagt
    12541 tcgccttttc atcccaccgg gccaggatca cgtccgcatc gcagtgcatg tccttcacct
    12601 ggtcgcggaa gaagccgaag gccaccatgc cgctatgttc gccgaggaac gccagttgct
    12661 tcgcgctggc gatcgcgccg acgccgccgg ccaaaaccga cgccatcacc cagccgacga
    12721 accagaagct ggcatgcttg cggttgacca ccgcacgcgc agccgcgacc aggacaacgg
    12781 ccaagctgcc gaccagggcc atgacgaccg tgatccggcc gttgtggaaa gcgatgggct
    12841 tgccagcgtc cgcttgcacg gcgtcgtaaa tgctggaccc gatgggcgcg cacatcagca
    12901 cgacaggcag cagcaccagg aacatcgtcc gcgtccattg cgcgagtgcc ttgcggcgtt
    12961 cgccggcggc aagcgcctcc atcatcggcg tgaagcccaa cagggccacc gcagccgcca
    13021 agccggcaac gatgccgcag gcgattacat acatacatcc tccctaatgc gccttgcgca
    13081 cggttgtagt cagagtccgg gta
//
