LOCUS       sequence-8107-ex       13116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 13116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1156
FEATURES             Location/Qualifiers
     source          1..13116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             186..899
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(216..244)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     839..859
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             922..2733
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             complement(2781..3929)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      4597..5307
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(5794..6444)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             6550..7749
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(8273..9064,9065..9133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI
                     ELDLNNGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATSLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGISAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9134..9238)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            9415..9523
                     /note="incP origin of transfer"
     protein_bind    9711..9732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9747..9752,9753..9770,9771..9777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9785..9801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9790..9812
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9809..9825
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9809..9825
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccatggatcc aagcttgctc aatcaatcac cggatctaga tatcgattaa ttaagtcgac
       61 atctagagtt taaactttaa ttaagcccgg gctgcagccc gggggatcca ctaggttaac
      121 gcatgcgggc ccggtaccag gcctagatag atagagagag agagagacta gtggaggaag
      181 aaaaaatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      241 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      301 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      361 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      421 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      481 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      541 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      601 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      661 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      721 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      781 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      841 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
      901 gagctctaga ctgaggtaat catggtccgt cctgtagaaa ccccaacccg tgaaatcaaa
      961 aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt
     1021 tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat
     1081 cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc
     1141 tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat
     1201 tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca
     1261 tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt
     1321 gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac
     1381 ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat ccatcgcagc
     1441 gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc
     1501 gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc
     1561 gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact
     1621 ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc
     1681 gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca
     1741 gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt
     1801 ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg ataacgtgct gatggtgcac
     1861 gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac
     1921 gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct
     1981 gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa
     2041 ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa
     2101 gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa
     2161 ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt
     2221 aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc
     2281 gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc
     2341 caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag
     2401 gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg
     2461 cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat
     2521 caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat
     2581 tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc
     2641 gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc
     2701 ggtgaaaaac cgcagcaggg aggcaaacaa tgactgcagg tggctgctga acccccagcc
     2761 ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg
     2821 tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac
     2881 ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg
     2941 tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg
     3001 cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc
     3061 tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg
     3121 tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc
     3181 gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag
     3241 cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc
     3301 ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg
     3361 ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc
     3421 tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc
     3481 atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg
     3541 atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc
     3601 aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc
     3661 atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc
     3721 gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta
     3781 gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg
     3841 cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg
     3901 tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccggaattc
     3961 cccggatcga tccgtcgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     4021 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     4081 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     4141 agctacctgc tttttctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     4201 attcatccgg ggtcagcacc gtttttgcgg actggctttc tacgtgttcc gcttccttta
     4261 gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gacggatcga tccggggaaa
     4321 ttaattcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg
     4381 taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca
     4441 ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca
     4501 gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta
     4561 ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt
     4621 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
     4681 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgccagg gcgcagccct
     4741 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
     4801 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
     4861 caaatgtcaa taggtgcgcc cctcatctgt catcactctg cccctcaagt gtcaaggatc
     4921 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
     4981 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
     5041 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
     5101 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
     5161 ccaagttttc cgcgtggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
     5221 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
     5281 ccagcccggt gagcgtcgga aaggcgctgg aagccccgta gcgacgcgga gaggggcgag
     5341 acaagccaag ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag
     5401 aatttccctg cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag
     5461 agccttgagt ccacgctaga tccgtcaatt cgatctatct catctgcgca aggcagaacg
     5521 tgaagacggc cgccctggac ctcgcccgcg agcgccaggc gcacgaggcc ggcgcgcgga
     5581 cccgcgccac ggcccacgag cggacgccgc agcaggagcg ccagaaggcc gccagagagg
     5641 ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct
     5701 gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt
     5761 agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc
     5821 aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg
     5881 ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg
     5941 cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg
     6001 ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc
     6061 cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg
     6121 cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc
     6181 ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg
     6241 caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg
     6301 cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt
     6361 gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac
     6421 tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc
     6481 accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg
     6541 aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac
     6601 gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac
     6661 tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt
     6721 gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc
     6781 gtctcgctgg ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg
     6841 gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc
     6901 gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc
     6961 gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc
     7021 tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc
     7081 tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc
     7141 aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg
     7201 gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc
     7261 gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt
     7321 ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg
     7381 cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg
     7441 acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg
     7501 ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa
     7561 ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg
     7621 atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc
     7681 tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc
     7741 gatcgctgat cgtggaaacg atagggaatt gacggatcta tctcatctgc gcaaggcaga
     7801 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc
     7861 ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag
     7921 aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg
     7981 gctgctacgg gcgtctgacg cggtggaaag ggggagggga tgttgtctac atggctctgc
     8041 tgtagtgagt gggttgcgct ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc
     8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8401 cactgatacc gcgagatcca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgatgttg
     8581 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8641 gttcccaacg atcaaggcgg gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8881 cggcgtcaac acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg
     8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gttgttgaga tccagctcga
     9001 tgtaacccac gcgtgcaccc aacttatctt cagcatcttt tactttcacc agcgtttctg
     9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9241 catttccccg aaaagtgcca cctgggtcga tcgacggatc ttttccgctg cataaccctg
     9301 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     9361 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     9421 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     9481 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct ctaagaaacc
     9541 attattatca tgacattaac ctataaaaat aagcgtatca cgaggccctt tcgtcttcaa
     9601 gaattaattc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9661 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9721 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9781 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9841 ctgatccccc gatgtgtagc agtggcggac catataggca gatcagaagg cgcggttctc
     9901 ctacatgttc gccggtgaac gcgttgagga agccgggcag tgcctcggca aaatccttgc
     9961 gtgtagacaa gacatctgcg tagcagttgt cctcaacaac gatgtcgaaa tccaaatcgg
    10021 agtgctcatc gagtcctccg tgaacgtaag agccgccgat cagaagagcg cggaagcgaa
    10081 catcggaagc gaccgcatcg cggatgcggt tcaagaaagt tgcatgagct tgtggaagtg
    10141 tgctgagcat aaatgattct cctagctgtt ctttgggtaa gtacgccatc aggacgttgt
    10201 gagtggcgcg atttttagcg gctgaaatca gcccttgagc ctgtcggcaa gtcgcgtcat
    10261 gaggtccatg cgctcatgca ggatcgccac gaccaacgcg ggttcgcccg cacgcggcag
    10321 gcaaaaaacg tagtggtgtt cgcagcgggc catccgcagc gcgggaaaga gttcgctcat
    10381 gtccttaaac gggccttcgc cggcggcaag cctggctatg ccctgttcca gcttagcgat
    10441 atagcggcgc acctgcgccg cgccccactc ccggcgcgtg tagcggatga tgccgcgtag
    10501 atcggcttcg gcctcagccg tgaggatgta ggccgtcaag cgcgatcccc gctgagttct
    10561 tcatcaagaa tttcgccgac gctcttggtg gacaccttgc cggcaagccc atcgttgatg
    10621 cggttcccca gcatggtttt cagttcctgc catgcctgat cggcatcagc gtcaccgggg
    10681 aacagacgtt cgagggcgta ttgcttaatg gtcttgccct gcaaggcggc cagggctttc
    10741 aggctctggt gctgctggtc cgtcatgtcg attgtcaggc ggctcattgg ataacctcca
    10801 taaaatacac gtaaccacat tagcacatat gtgggcgtga ggctacagcg cgaggcgcat
    10861 taaggtcggg aaaatgcgct aggcgcattt aaattgcgta ttgctgtaat gcgccatgcc
    10921 ggctagacta ggcccaaatg ggtataccca atttgaccaa gggggacgcg atgagggcgg
    10981 ccaagcacta ccgacaactt ctatccatcg acttcaacat cgaggcgctg gccttcgtgc
    11041 ctggacccga cggcacacgc ggccggcgca tccacgtcct ggggcgcgag gtccgcgacc
    11101 ggcccggcct ggtcgagtac ctttcgccgg cgttcggctc gcgggtggcg ctggacggct
    11161 actgcaaggc caatttcgat gcagtgctgc acctggcgta ccccgatcat cagcaatggg
    11221 gccacgcatg aagcgccgaa gctacgccat gctgcgcgcc gctgccgcgc tggccgtcct
    11281 ggtcgttgcc tcgccggcat gggccgagct gcgcggcgag gtcgtgcgca tcatcgacgg
    11341 cgacaccatc gacgtgctgg tagacaagca gccggtgcgc gtgcgcctgg tggacattga
    11401 cgcgccggaa aagcggcaag ccttcggcga acgtgcgcgc caggcgctgg ccggcatggt
    11461 gttccgccgg cacgtcctgg tcgacgagaa ggacaccgac cgttacggcc gcacgctggg
    11521 caccgtgtgg gtcaacatgg agctggccag ccggccgccg cagccgcgca acgtcaacgc
    11581 cgcgatggtt caccagggca tggcgtgggc ctatcgcttc cacggccgcg cggccgaccc
    11641 tgaaatgctg cggctcgaac aggaggcgcg aggcaagcgc gtcggcctct ggtccgatcc
    11701 gcacgccgtc gagccgtgga aatggcgacg cgagagcaac aaccggaggg acgaaggttg
    11761 aaggtcgccc gcatctacct gcgcgccagt acggacgagc agaatcttga acgccaggag
    11821 agccttgtag cggccacgcg ggccgccggg tactacgtcg ccggcatcta ccgcgagaag
    11881 gcgtccggcg cacgcgccga ccggcccgag ctgctgcgca tgatcgcgga cctgcaacct
    11941 ggtgaagtcg tcgttgcgga gaagatcgac cgcatcagcc gcttgccgtt ggccgaggcc
    12001 gagcgcctgg ttgcgtcgat ccgggccaaa ggggccaagc tggccgtgcc tggcgtggtg
    12061 gacctgtcgg agctggccgc cgaggcgaac ggagtggcga aaatcgttct ggaatccgtc
    12121 caggacatgc ttttgaagct cgccttgcag atggcccgcg acgactacga ggatcggcgc
    12181 gagcgtcaac gtcagggtgt ccagttggcg aaggccgccg gccgctacac cggccgcaaa
    12241 cgtgacgccg gcatgcacga ccgcatcatc acgcttcgct ccggcggatc gagcattgcc
    12301 aagacggcca agctggtcgg atgcagcccg agccaggtca aacgagtgtg ggcggcctgg
    12361 aacgcgcagc agcaaaaata aagccgggca gtgcccggct tttctcacct tttcgcgtcc
    12421 cgcagggccg ctgcgagcgc cctacctaga tcctcgcttt ccccctcggt gtagtccggc
    12481 cagggcacga agggcgcgga tgcgaacctg ttgagcaggt acgccttcgg gcagcggtag
    12541 accaccggcg agttcgcctt ttcatcccac cgggccagga tcacgtccgc atcgcagtgc
    12601 atgtccttca cctggtcgcg gaagaagccg aaggccacca tgccgctatg ttcgccgagg
    12661 aacgccagtt gcttcgcgct ggcgatcgcg ccgacgccgc cggccaaaac cgacgccatc
    12721 acccagccga cgaaccagaa gctggcatgc ttgcggttga ccaccgcacg cgcagccgcg
    12781 accaggacaa cggccaagct gccgaccagg gccatgacga ccgtgatccg gccgttgtgg
    12841 aaagcgatgg gcttgccagc gtccgcttgc acggcgtcgt aaatgctgga cccgatgggc
    12901 gcgcacatca gcacgacagg cagcagcacc aggaacatcg tccgcgtcca ttgcgcgagt
    12961 gccttgcggc gttcgccggc ggcaagcgcc tccatcatcg gcgtgaagcc caacagggcc
    13021 accgcagccg ccaagccggc aacgatgccg caggcgatta catacataca tcctccctaa
    13081 tgcgccttgc gcacggttgt agtcagagtc cgggta
//
