LOCUS       sequence-81071-e       13418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP reporter for fli-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Lu J, Dentler WL, Lundquist EA
  TITLE     FLI-1 Flightless-1 and LET-60 Ras control germ line morphogenesis in
            C. elegans.
  JOURNAL   BMC Dev Biol. 2008 May 16;8:54. doi: 10.1186/1471-213X-8-54.
  PUBMED    18485202
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-05-16"; volume: "8:54. doi"; pages: " 
            10.1186/1471-213X-8-54"
COMMENT     Sequence Label: pEL392
FEATURES             Location/Qualifiers
     source          1..13418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9088..9116)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9864..9884
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(10858..10880)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10980..10999
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11077..11095)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11163..11267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11268..11336,11337..12128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11486..11505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12299..12887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12788..12807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13041..13058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13175..13196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13211..13216,13217..13234,13235..13241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13249..13265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13254..13276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13273..13289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13273..13289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcagtt tagacaggca ctttcgactg taaactaaaa ttttcaaaat
       61 agtttcaaaa gtattactac gatatctagt catttttatc ttctatgaca tgttccaaaa
      121 ctttctgata aacacgaact ttatctttat gctcaatagc tgcacattct tcatcctttg
      181 gcttttcatc ttctccagta ttatcgtttt ccgttgttcc ggtcatctga atacacacac
      241 atcatttcca tatgaataac aagagataaa acagagaaaa agaaggacaa agatgcgatt
      301 caaagattcg aaaggttgcg acttcagttg gtacttctct ggcatttctt ccctcctctg
      361 atatatccgg tccaccttgc ccgtcaaatg ctctctaata tctgcgtctc tctgggttcc
      421 cgtgctattt ttcatggatc tgtagtgtaa cactcatcgg gcgagttgtc tgtgtctcta
      481 ttccgatcac tctctctcta cagcttctcc tttctgttcg tgtgaggctc ccgggccggg
      541 gtgctcgtcc cgtttcttta tgtttctccg tttcggatca ccttgttaat tgttcgtctc
      601 ccagttccac ttcttgctct ttttgggttt attcgttata cgaattttca aagtttcaca
      661 gaacaacctt taaatttttt aaactttaac agttgcacat aaccatttta aaatctcgac
      721 ttcaattcct aggaaaatcc ttaaaatata ttggttcttg aacctaatgt acagtagcta
      781 ctactgtagc taaacagtaa taaaattgcg aaaattgtta ccaatttgat atgttttcct
      841 atttcacttc atttccaaaa gtcagcagtt atcacttact tttatttcga aatatttttt
      901 tataaaaaag atttttgatg agaaaaccag cacggaaatg caaaataaag tcaggtgaca
      961 aaatgttgta aacacaaatg atgcccaata attatgaaat aggaggtatt ctttttatat
     1021 ttgacacact tctgatatca cttccttttc gtgctcaaaa ccaattttaa tgaagaaaaa
     1081 atgtggtaaa atctcataaa tcttgcattc tagacttgaa attttcactt ctcttttcga
     1141 acggttattt ttgccacgcg caaatcctag gccacggcct tccggtttct aggccaccac
     1201 cgccattttg agttttttca attacaaatt taacaatttc cacagttttc tttgaaattt
     1261 attcatttaa ttgctttaaa tctatattta tatattaatt ttagtgttta gtgaaataaa
     1321 atgcaataga tcgctacaaa tatgtcaaca ggagtgttac aattcgtcaa aggaatcgat
     1381 ttctcgggaa atgacttttc ggtgagtttt tcttagtttt agacacaatt tttatacgtt
     1441 ttttttcagg gagatcgatt tcctcacgat gtggaacaaa tgacgcaaat gacatggttg
     1501 aaattgaacg attcgaagct cgaacaggtg cctgatgagc tgtcgcgttg tgcgaatctg
     1561 gaacatctgc aaatggctca caatcaactt atttctgtac atggagagct ttctgattta
     1621 ccgcgactac gatcagtgat tgtcagggat aataacgtaa gaatttttgt tttctaattt
     1681 agttaaaata aaataaaacg agattttcag ctaaaaaccg ccggaattcc aacggacatt
     1741 ttccgcatga aagatcttac aattatcgat ttatcgcgaa atcaattgag agaagttcca
     1801 acaaatttgg agtatgcaaa aggatcaatc gttctgaatt tgagctataa taatattgaa
     1861 actattccga acagcgtttg tgccaatctt atcgacttat tgttcctgga tctttcgaac
     1921 aacaaattgg acatgcttcc accacagatt cgtcgattaa gcatgcttca aagtttgaaa
     1981 ctttcaaata atccacttaa tcatttccaa ttgaaacaat taccaaggtt actgaaaaaa
     2041 aacagaaatt caaaacaaag agtctttcag catgacatcc ctaagcgttc ttcacatgag
     2101 taataccaat cgaacgctgg ataatattcc tccaactctg gatgatatgc ataatttaag
     2161 agtgagtttc agagttttca gcttacaatt taaagtttta cgtttccagg atgttgattt
     2221 ttctgaaaac aatcttccca ttgtcccgga agctctcttc aaactgcgaa atcttcgaaa
     2281 attgaatcta agtggaaata aaatcgaaaa attgaatatg actgaaggag aatgggagaa
     2341 tttggaaaca ttaaacatga gccataatca attgacagtt cttccggatt gtgttgtcaa
     2401 attgacaaga ctcaccaaat tatacgcagc aaataaccaa ttgacattcg aaggaatacc
     2461 atctggaata ggaaaactca ttcaacttac cgtacttcat ttgtcatata acaagctgga
     2521 attagttcca gaaggaatct cgcgatgtgt taagcttcag aaattgaaat tagatcataa
     2581 tcgattgatt acactgccag agggaataca tttgttgcct gatttgaaag ttttggattt
     2641 acatgagtaa gtttgaaatt taaaagtatt aagatattga aaaaaaagtt tttaaatttt
     2701 taaaaaaatt ttgttgcgga aattattggc ctagcttcgt tatgacaaaa atcaaaaaat
     2761 ttagctcaaa aagcgaaaat tacgaaacta ggcctataat ttccacgaaa attcaaaaag
     2821 aattcagcta ttttgagtat tttttttgtt gaaaaattga ataagcacag aaaaatgaat
     2881 aaaacaatgt tttaagcaaa ttgataacta gaaaagcgtt gccgaacaaa attcttccgt
     2941 aaaagtctcg agactttttt tctcacaatt tatcaatggt tatacgcttc aattctttta
     3001 tatattttga tatgcaaaca ttttgaaaaa gccgaaaaaa aataatgtgt tcttttattt
     3061 ttttttggaa atttctcatg cctctctcgg aacacatgac tcaacattac tagagacaca
     3121 acaacagata tgaaatttcc caaaattgag aaaaagattt gcattaatga agttgtggcc
     3181 attgggatta atcgatggtg gtcaacttct tcatttgaat catcggattt ttcttttaaa
     3241 atttttaatt ttcagaaacg aaaacttggt gatgcctcca aaaccgaatg atgcaagaaa
     3301 gaaattagca ttttataata ttgatttctc gttggaacat caggtttgtt ggaatttaaa
     3361 aaatgattga gaaaaagaca tattgacgtg aaaatataat ttcaattttt tcggtttttt
     3421 tccagagaac atcgattttc aaatttcgaa gaacaaaaag tgtgattttt aaaacttaat
     3481 tgaaaatcat tcaacagttt atcaaaaaaa aaacattcct ttcaaccaga aaccgatatt
     3541 ttaagctctt ttcgaaaaat ataagtttct acgaaatata acttctaaaa aagaacattt
     3601 tcgattttgt tttaaaaatg aaaaaaaaag ataggaaatt attgactatt tgaattattt
     3661 tttcaagaaa attcgaaaaa catgagtact gtatttctgc gtcgaaactc gcataattta
     3721 taaatttata aaaatattag cggacactaa aatattttaa tatataaaac cagtcacaaa
     3781 tttccaaaaa tttgcggttt tgtaaacgaa aaaatatttt ttttttcaaa atactgcaaa
     3841 ataatttttc atgccaaaaa tcaatgttta accattttca ttcataaatc tatcaatttc
     3901 gagtaaacac aattcatttc gaatgcatta ctgctccatc ttccagttct ttctgtaaaa
     3961 tttgaaaaat tgcatcattt tacatttttg atctacctca tttttcaaga aaaagttcgc
     4021 gcgttggggg gtttccttct ttatttttgc aaaaaatgag tcgttcactt cctcccattt
     4081 tttgcacttt tttctatccc attttggtca gttgccggaa aaaaaacgac atcttatttc
     4141 ttctcttctt ctcaatttct tcctccacat cttcttctcc tcttactttg tcttgttccg
     4201 gtcggagagc ccgctttgaa aactcgtaga gagcagtctt aaagcagaaa agaagagcag
     4261 agcataactt tcgaaaaaaa aatctgcaaa aaaatattcg ctaggccatt ttcaatacat
     4321 atttgatatt tctattgaat ttcgtttaaa aaaaacaatg ctgctggcag attgtctttg
     4381 ggtgagtgac ctcgaaccag caggtgatca acagatggaa aaaagtttta aagaattatt
     4441 tatttagata ttgaaatatg aattgtaaag caaaaaatat ctagaatttg gttccaaatt
     4501 agctgtaatc aattttattt acactttttt tcgtaagaaa atcatgtaac caaaaaagat
     4561 agaatcatat ttatttattt attgcaaaaa gtcacgtttt ttgaaaactt gaattaaatt
     4621 tagtttttaa aatttagcat ttcttatttt aatttctata taggaattta ttttctaatt
     4681 aaactttttt cagcgtaaaa tcgcagggca aatgacaagt ccatcgtctt cgatctcatc
     4741 agttcattcg ggtggagcac gacaagatgc tttggcacga cggaaagaat ttattcgacg
     4801 cagaaagcaa caggccgatc aacagagtgc tgataaggtt atacaggtga gagatttaga
     4861 aaaaaaaaat acgcagagtg gtcctaactt ccgataaatc aaaaaaggag aaaatacaaa
     4921 aatttgaaaa acaaaacgtt aaacgaattt tcaatttttg ataatcttca tttgaaatac
     4981 tgcccttttt tccgtgccgt gaagcagttt aaaacggaag cagaattcaa ttttcttgaa
     5041 aatttctgtg aaacctataa aaatttccag aaaattgtac tgtgatatga ttcataaaac
     5101 atcgaatctc atattccaat acaaaaaaat ttgcataata atttttcgaa acggaattgt
     5161 tcttataaaa atgaattctc tcatcgataa tctcgaccca taaactatcc tcccgttgat
     5221 atcatctttt agttgttttt attcctcttg tgagaattat cctattaata tcacatcagt
     5281 agatcagttc cttcatttct ttcttttttt ttgggttgtg ttttggtttt gaaagtgaga
     5341 aagtaacaat caaggttcaa ggttggcatc gtctgaaaaa gaaaacgatt ttgacatttt
     5401 tgaataaaac aacgttgaat ttttccattt cttctctttt aatttttaat ttttacaggg
     5461 aatgtcgaaa atcgctggcg tcggtgcggc tcttacggag aagcaacaag aggaagaaga
     5521 acgacaattg gaagcaaaaa gtgcagttaa ttggaaaaag aatattgaaa agaatcgtcg
     5581 acacattgat tacagtgata tatttgatga agatgttggt agtgacgaag gaatgtgggt
     5641 atgggaaatt gagaatttct atccatcaat tatggatgaa gcattccatg ggcaatttta
     5701 tgatgcagat gcttacctgg tactaaagac gactcgagaa gcatccggac aacttcgtca
     5761 tgcaatattt tattggctcg gtgagcacgc ttctcttgac aagggaatgt gttctgctgt
     5821 acacgctgtt ggcctccgaa atcacctgaa tgccacgtgt cgaacacaac gagaagaaat
     5881 gaacgatgag acggaagagt ttttgacgtt atttggagaa gaaattgtgt atattgaggg
     5941 tggacggaca atttctggat tctatacaac tgaaaaacca gctcatttaa cacgactata
     6001 tcgagcagga gttaacggaa ctgctgtaga aatggaaccc gtaccgctca gtgtcgaatc
     6061 attagatcct agattttgct ttcttttgga tgcaggagaa actatttgga tatggagtgg
     6121 ctataaatca agggtaataa tcagttttat tcgctaaaaa tcaaatatga caaacgattt
     6181 gataattttc ccattatctt atttatggag gggtactgta gtttgacttt tcatctcatt
     6241 gaatatgtag tattgcaaaa tcgtttcaga acatgagata aaaaatttta aaaatcctaa
     6301 aattgaaatg atgaaataac gcattttctc gccattctcc tcttttaaaa attgagagat
     6361 cctttgagac ggagagaccg agagcactct cccccatttc tcaccactct cccttctcct
     6421 actgcatgtc ttctatgtaa actgcatcct ttcctcttgt tttcgtgact gcttggcgca
     6481 atggtagcgc gttcgactcc agatcgaaag gttgggcgtt cgatccgctc agtggtcaaa
     6541 tgttttgttt ttgagcttta agtttggttc tttcagttat ggtttttttt gcagatcaca
     6601 gtgtcaaata aagcaagatt gttcgcagag cggctcaaca agcgtgatag gaaaggaaag
     6661 agtgaaattg agacatgccg acaagctaga tgtcctccag aattctggca agcgctcact
     6721 ggaaatccgg ataaaccaca aggagctatt gttgaacatg taccggaagg atttgttgct
     6781 gaaagaaaaa agctttataa ggtgaacatt ggaatgggat tcctcgaatt accacaagta
     6841 gagctgccca agggaattgc aaaacaagat atgcttggat ccaaaggagt tttcgttctt
     6901 gattccaatt cggacatctt tttatggatt ggcaaaaaag cgaaccgatt actgaaaatg
     6961 gctggtcaga aattagttgt tgaactacat caaatgattg atagaccaga ttatgctcaa
     7021 gtatatcgag aaactgaagg agaagaatca atgatgttcc gatcgaaatt tgctggatgg
     7081 gatgaaatag ttccagttga ttacacgaga acaagtgatt ctgtacaaag agttccagat
     7141 ttgaaggtta ttgtgaagaa agataatatg atgcgtgcag atttggcagc tctattccta
     7201 gaaagacaac cttcaatgtc atatgaagaa agtgaagaac taatggaaga ttgcaattat
     7261 gatttagagc ttatggagag ttttgtactt gagggaaaga aattcgtgaa acttccacaa
     7321 aaagagtttg gaatattcta cacaatggac tgttacgtat tcctttgtcg atatgcagta
     7381 atgccggaag aggatgagga aggtgaagat gaacacgatg aagatgataa gccagagatg
     7441 gacttcaagt gtgtcgtgta cttttggcaa ggaagagatg cttcaaatat gggatggttg
     7501 aattttacat tccagcttca accaaatttt gaggagatat ttaaggataa attggaagta
     7561 tgttaaaaca aaattataga ttatcatttt caaattcaat ttcaggttgt tcgaatgtat
     7621 caacaacaag agaatcataa attcctttcg catttcaaac gaaaattcct gatcaagcgt
     7681 ggtcgacgtg gtttgacaaa gaatctcggc ggaaaatggc cagaattatt ccaaatgaga
     7741 gccaatggat caagtgtatg caatagaact attcaagttg attgccagtg agtttcatgg
     7801 atttttgaat aaaaatttcc ctaataaatt tcaaaaaata gctcattttg agaccaaata
     7861 ccgtatttgt gaagtaaaaa tcgaaaaatt tagcgtctct gtaatagaag gcaacacaaa
     7921 tattttaatt cattttttat aattcagagc aaaccaacta tgctcagcat tctgtcacat
     7981 gctccgaatt ccattcaaag aaatcgaaga agatggtcat cgtggagtgg tttacgtatg
     8041 gatgggtaaa gattctgatc cacgtgaaca tgaatttgct cgacaagtag catcagatct
     8101 tgtcgttcgt gacgatgaca atgacttccg aattgtcgaa gttcaagaag gagaagaaaa
     8161 tgaagaattc tggaaagttc tcggtggaaa gaaaaaatat gaaactgata gttcatttgt
     8221 taagcataca agactgtttc gatgcaccaa cgagaaggga tactttgcga tttccgagaa
     8281 aactgtcgat ttttgtcagg taattttagt aaaataattt tttaaaatat gttttcgggt
     8341 attagcagtg aaactttatc agctaccgta cccttcattt agttcttatt cctctctgaa
     8401 aattttactt tttcgtcaaa atttaataaa attttttaaa ttaatttccg gtcagaaatt
     8461 tttgaagtat taaaacttaa aaatttaatt tgaattttaa aaaattttag ctagaattat
     8521 tagaaaattt aaaatctaaa aaaattcgga aaatcttaca aactgaaata tttcaggatg
     8581 atcttgatga tgacgatata atgattctgg ataacggaga tgcagttttc ctttggattg
     8641 gagcaagatc ttcagatatt gaagcaaaac tttcttatca agccgctcaa gtatatcatg
     8701 catcaatgag aatgaaagca aatgagaagc cgagaaagtt catgttagca gttcgaggac
     8761 gagaaagttg cagattccgg aaatgcttcc atgcatggtc caaaatgaag gttcgtttga
     8821 aagaaatatt gggtacttca tttaataatc caaccagaca cgaagtatac cctagtttat
     8881 ttaaaaatgt tatcacaatc cacaaaataa attcttattt tttaggaacc aatgggcctg
     8941 caggtcgact ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     9001 atactaacat aacatagaac attttcagga ggacccttga gggtaccggt agaaaaaatg
     9061 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     9121 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     9181 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
     9241 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
     9301 tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc
     9361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     9421 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     9481 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     9541 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     9601 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
     9661 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     9721 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     9781 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     9841 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     9901 catggcatgg atgaactata caaatagcat tcgtagaatt ccaactgagc gccggtcgct
     9961 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
    10021 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
    10081 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
    10141 caccccctat ttttgttatt atcaaaaaaa cttcttctta atttctttgt tttttagctt
    10201 cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt aattcgtaat
    10261 aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat cccaaaatct
    10321 acacaatgtt ctgtgtacac ttcttatgtt ttttttactt ctgataaatt ttttttgaaa
    10381 catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc
    10441 gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga
    10501 aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt
    10561 cctgcttttg ctttttgggg gtttccccta ttgtttgtca agagtttcga ggacggcgtt
    10621 tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt
    10681 gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct
    10741 atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat aactgtttcc
    10801 tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10861 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10921 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10981 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11041 cgtaaggaga aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat
    11101 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    11161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    11221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    11281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    11341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    11401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    11461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    12001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    12061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    12121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    12181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    12241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    12301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    12361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    12421 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
    12481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12661 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
    12721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    13021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    13081 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13141 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    13201 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    13261 aacaatttca cacaggaaac agctatgacc atgattacgc caagctgtaa gtttaaacat
    13321 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
    13381 tcacaacgat ggatacgcta acaacttgga aatgaaat
//
