LOCUS       sequence-8108-ex       13079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 13079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1161
FEATURES             Location/Qualifiers
     source          1..13079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             186..863
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             186..863
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(316..334)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     584..603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     778..801
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             885..2696
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             complement(2744..3892)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      4560..5270
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(5757..6407)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             6513..7712
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(join(8236..9027,9028..9096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI
                     ELDLNNGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATSLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGISAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9097..9201)
                     /gene="bla"
                     /label=AmpR promoter
     oriT            9378..9486
                     /note="incP origin of transfer"
     protein_bind    9674..9695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9710..9715,9716..9733,9734..9740)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9748..9764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9753..9775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9772..9788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9772..9788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccatggatcc aagcttgctc aatcaatcac cggatctaga tatcgattaa ttaagtcgac
       61 atctagagtt taaactttaa ttaagcccgg gctgcagccc gggggatcca ctaggttaac
      121 gcatgcgggc ccggtaccag gcctagatag atagagagag agagagacta gtggaggaag
      181 aaaaaatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
      241 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
      361 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca
      421 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      481 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      541 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      601 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg
      661 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
      721 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc
      781 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
      841 gccgccactc caccggcgcc taagagctct agactgaggt aatcatggtc cgtcctgtag
      901 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
      961 aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg
     1021 ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca
     1081 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     1141 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     1201 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     1261 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     1321 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     1381 actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     1441 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     1501 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     1561 ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     1621 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     1681 cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     1741 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat
     1801 tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct
     1861 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     1921 tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg
     1981 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc
     2041 aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     2101 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     2161 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     2221 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     2281 accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     2341 aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     2401 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     2461 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     2521 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     2581 acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
     2641 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatgactgc
     2701 aggtggctgc tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca
     2761 ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg
     2821 cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc
     2881 ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc
     2941 gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc
     3001 cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc
     3061 cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt
     3121 cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt
     3181 aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga
     3241 tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt
     3301 catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga
     3361 aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg
     3421 cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga
     3481 acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct
     3541 tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca
     3601 tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac
     3661 gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc
     3721 tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt
     3781 cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc
     3841 ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc
     3901 cttgcgggat tgccccggaa ttccccggat cgatccgtcg atcttgatcc cctgcgccat
     3961 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     4021 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     4081 tatcgccatg taagcccact gcaagctacc tgctttttct ttgcgcttgc gttttccctt
     4141 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttttg cggactggct
     4201 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa
     4261 gctgacggat cgatccgggg aaattaattc acccccgaac acgagcacgg cacccgcgac
     4321 cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt ccgtgaatgc
     4381 cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac tgcccggcac
     4441 ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg gcgtcgcgct
     4501 cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga ctgccagcgc
     4561 tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg gggatgggag
     4621 gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc ggcgtgcgcg
     4681 gtcacgcgcc agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc
     4741 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga
     4801 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcatcact
     4861 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg
     4921 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta
     4981 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg
     5041 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc
     5101 cgcccctcat ctgtcagtga gggccaagtt ttccgcgtgg tatccacaac gccggcggcc
     5161 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
     5221 cgccagccca gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc tggaagcccc
     5281 gtagcgacgc ggagaggggc gagacaagcc aagggcgcag gctcgatgcg cagcacgaca
     5341 tagccggttc tcgcaaggac gagaatttcc ctgcggtgcc cctcaagtgt caatgaaagt
     5401 ttccaacgcg agccattcgc gagagccttg agtccacgct agatccgtca attcgatcta
     5461 tctcatctgc gcaaggcaga acgtgaagac ggccgccctg gacctcgccc gcgagcgcca
     5521 ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac gagcggacgc cgcagcagga
     5581 gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc tagggcaggg
     5641 catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag ggggagggga
     5701 tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa
     5761 tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt cgccaatcca
     5821 tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg
     5881 tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg
     5941 ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa gtagctgatt
     6001 gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc
     6061 tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg
     6121 ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat cagaaatgag
     6181 cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag catggcttcg
     6241 gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga
     6301 acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac
     6361 aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct tgacacttta
     6421 tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg cgcgttcaat
     6481 cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacataccc ctgatcgtaa
     6541 ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg gtgctgccgg
     6601 gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc attctgctgg
     6661 cgctgtatgc gttggtgcaa tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt
     6721 tcgggcggcg gccaatcttg ctcgtctcgc tggccggcgc cactgtcgac tacgccatca
     6781 tggcgacagc gcctttcctt tgggttctct atatcgggcg gatcgtggcc ggcatcaccg
     6841 gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc gatgagcgcg
     6901 cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc
     6961 tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg gcagccttga
     7021 acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc
     7081 ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga
     7141 ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga caggtgccgg
     7201 ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc acgatcggca
     7261 tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc accggccctg
     7321 tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc gacggcacag
     7381 gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc atggtcctgc
     7441 ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg caggtggatg
     7501 aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg acctcgatcg
     7561 tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg aacgggtggg
     7621 catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt
     7681 ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggga attgacggat
     7741 ctatctcatc tgcgcaaggc agaacgtgaa gacggccgcc ctggacctcg cccgcgagcg
     7801 ccaggcgcac gaggccggcg cgcggacccg cgccacggcc cacgagcgga cgccgcagca
     7861 ggagcgccag aaggccgcca gagaggccga gcgcggccgt gaggcttgga cgctagggca
     7921 gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg acgcggtgga aagggggagg
     7981 ggatgttgtc tacatggctc tgctgtagtg agtgggttgc gctccggcag cggtcctgat
     8041 cccccgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8341 cttaccatct ggccccagtg ctgcactgat accgcgagat ccacgctcac cggctccaga
     8401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8521 taatagtttg cgcaacgatg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     8581 tggtatggct tcattcagct ccggttccca acgatcaagg cgggttacat gatcccccat
     8641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8821 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcac cacatagcag
     8881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8941 accgttgttg agatccagct cgatgtaacc cacgcgtgca cccaacttat cttcagcatc
     9001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgatcgacgg
     9241 atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     9301 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     9361 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     9421 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     9481 tggccgataa gctctaagaa accattatta tcatgacatt aacctataaa aataagcgta
     9541 tcacgaggcc ctttcgtctt caagaattaa ttccaatacg caaaccgcct ctccccgcgc
     9601 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9661 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9721 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9781 gctatgacca tgattacgcc aagctgatcc cccgatgtgt agcagtggcg gaccatatag
     9841 gcagatcaga aggcgcggtt ctcctacatg ttcgccggtg aacgcgttga ggaagccggg
     9901 cagtgcctcg gcaaaatcct tgcgtgtaga caagacatct gcgtagcagt tgtcctcaac
     9961 aacgatgtcg aaatccaaat cggagtgctc atcgagtcct ccgtgaacgt aagagccgcc
    10021 gatcagaaga gcgcggaagc gaacatcgga agcgaccgca tcgcggatgc ggttcaagaa
    10081 agttgcatga gcttgtggaa gtgtgctgag cataaatgat tctcctagct gttctttggg
    10141 taagtacgcc atcaggacgt tgtgagtggc gcgattttta gcggctgaaa tcagcccttg
    10201 agcctgtcgg caagtcgcgt catgaggtcc atgcgctcat gcaggatcgc cacgaccaac
    10261 gcgggttcgc ccgcacgcgg caggcaaaaa acgtagtggt gttcgcagcg ggccatccgc
    10321 agcgcgggaa agagttcgct catgtcctta aacgggcctt cgccggcggc aagcctggct
    10381 atgccctgtt ccagcttagc gatatagcgg cgcacctgcg ccgcgcccca ctcccggcgc
    10441 gtgtagcgga tgatgccgcg tagatcggct tcggcctcag ccgtgaggat gtaggccgtc
    10501 aagcgcgatc cccgctgagt tcttcatcaa gaatttcgcc gacgctcttg gtggacacct
    10561 tgccggcaag cccatcgttg atgcggttcc ccagcatggt tttcagttcc tgccatgcct
    10621 gatcggcatc agcgtcaccg gggaacagac gttcgagggc gtattgctta atggtcttgc
    10681 cctgcaaggc ggccagggct ttcaggctct ggtgctgctg gtccgtcatg tcgattgtca
    10741 ggcggctcat tggataacct ccataaaata cacgtaacca cattagcaca tatgtgggcg
    10801 tgaggctaca gcgcgaggcg cattaaggtc gggaaaatgc gctaggcgca tttaaattgc
    10861 gtattgctgt aatgcgccat gccggctaga ctaggcccaa atgggtatac ccaatttgac
    10921 caagggggac gcgatgaggg cggccaagca ctaccgacaa cttctatcca tcgacttcaa
    10981 catcgaggcg ctggccttcg tgcctggacc cgacggcaca cgcggccggc gcatccacgt
    11041 cctggggcgc gaggtccgcg accggcccgg cctggtcgag tacctttcgc cggcgttcgg
    11101 ctcgcgggtg gcgctggacg gctactgcaa ggccaatttc gatgcagtgc tgcacctggc
    11161 gtaccccgat catcagcaat ggggccacgc atgaagcgcc gaagctacgc catgctgcgc
    11221 gccgctgccg cgctggccgt cctggtcgtt gcctcgccgg catgggccga gctgcgcggc
    11281 gaggtcgtgc gcatcatcga cggcgacacc atcgacgtgc tggtagacaa gcagccggtg
    11341 cgcgtgcgcc tggtggacat tgacgcgccg gaaaagcggc aagccttcgg cgaacgtgcg
    11401 cgccaggcgc tggccggcat ggtgttccgc cggcacgtcc tggtcgacga gaaggacacc
    11461 gaccgttacg gccgcacgct gggcaccgtg tgggtcaaca tggagctggc cagccggccg
    11521 ccgcagccgc gcaacgtcaa cgccgcgatg gttcaccagg gcatggcgtg ggcctatcgc
    11581 ttccacggcc gcgcggccga ccctgaaatg ctgcggctcg aacaggaggc gcgaggcaag
    11641 cgcgtcggcc tctggtccga tccgcacgcc gtcgagccgt ggaaatggcg acgcgagagc
    11701 aacaaccgga gggacgaagg ttgaaggtcg cccgcatcta cctgcgcgcc agtacggacg
    11761 agcagaatct tgaacgccag gagagccttg tagcggccac gcgggccgcc gggtactacg
    11821 tcgccggcat ctaccgcgag aaggcgtccg gcgcacgcgc cgaccggccc gagctgctgc
    11881 gcatgatcgc ggacctgcaa cctggtgaag tcgtcgttgc ggagaagatc gaccgcatca
    11941 gccgcttgcc gttggccgag gccgagcgcc tggttgcgtc gatccgggcc aaaggggcca
    12001 agctggccgt gcctggcgtg gtggacctgt cggagctggc cgccgaggcg aacggagtgg
    12061 cgaaaatcgt tctggaatcc gtccaggaca tgcttttgaa gctcgccttg cagatggccc
    12121 gcgacgacta cgaggatcgg cgcgagcgtc aacgtcaggg tgtccagttg gcgaaggccg
    12181 ccggccgcta caccggccgc aaacgtgacg ccggcatgca cgaccgcatc atcacgcttc
    12241 gctccggcgg atcgagcatt gccaagacgg ccaagctggt cggatgcagc ccgagccagg
    12301 tcaaacgagt gtgggcggcc tggaacgcgc agcagcaaaa ataaagccgg gcagtgcccg
    12361 gcttttctca ccttttcgcg tcccgcaggg ccgctgcgag cgccctacct agatcctcgc
    12421 tttccccctc ggtgtagtcc ggccagggca cgaagggcgc ggatgcgaac ctgttgagca
    12481 ggtacgcctt cgggcagcgg tagaccaccg gcgagttcgc cttttcatcc caccgggcca
    12541 ggatcacgtc cgcatcgcag tgcatgtcct tcacctggtc gcggaagaag ccgaaggcca
    12601 ccatgccgct atgttcgccg aggaacgcca gttgcttcgc gctggcgatc gcgccgacgc
    12661 cgccggccaa aaccgacgcc atcacccagc cgacgaacca gaagctggca tgcttgcggt
    12721 tgaccaccgc acgcgcagcc gcgaccagga caacggccaa gctgccgacc agggccatga
    12781 cgaccgtgat ccggccgttg tggaaagcga tgggcttgcc agcgtccgct tgcacggcgt
    12841 cgtaaatgct ggacccgatg ggcgcgcaca tcagcacgac aggcagcagc accaggaaca
    12901 tcgtccgcgt ccattgcgcg agtgccttgc ggcgttcgcc ggcggcaagc gcctccatca
    12961 tcggcgtgaa gcccaacagg gccaccgcag ccgccaagcc ggcaacgatg ccgcaggcga
    13021 ttacatacat acatcctccc taatgcgcct tgcgcacggt tgtagtcaga gtccgggta
//
