LOCUS       sequence-81090-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains an N-terminal tandem affinity purification tag (relevant to
            any system) within a construct designed for genome integration into 
            trypanosomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Schimanski B, Nguyen TN, Gunzl A
  TITLE     Highly efficient tandem affinity purification of trypanosome protein
            complexes based on a novel epitope combination.
  JOURNAL   Eukaryot Cell. 2005 Nov;4(11):1942-50.
  PUBMED    16278461
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell."; date: "2005-11"; volume: "4(11)"; pages: "1942-50"
COMMENT     Sequence Label: pN-PURO-PTP
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..84)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             complement(91..111)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(148..321)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(322..495)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 cgactcacta tagggcgaat tgggtaccgg gccctacgta gcggccgctt tcccatcaat
       61 aagacgagga tccacctgat cttctagttc accttgaaaa tataaatttt cagaagcagt
      121 tgtaggtata tcacaattcg cgtctacttt cggcgcctga gcatcattta gctttttagc
      181 ttctgctaaa aggttagcgc tttggcttgg gtcatctttt aaactttgga tgaaggcgtt
      241 tcgttgttct tcgtttaagt taggtaaatg taagatctca tagaacgcgt tttgttgttc
      301 tttgttgaat ttgttgtcta ctttcggcgc ctgagcatca tttagctttt tagcttctgc
      361 taaaaggtta gcgctttggc ttgggtcatc ttttaaactt tggatgaagg cgtttcgttg
      421 ttcttcgttt aagttaggta aatgtaagat ctcatagaac gcgttttgtt gttctttgtt
      481 gaatttgttg tccacggctt catcgtggtg cgcaaggcct gccatgtttg cacgcttcac
      541 gctttccaca acttattctg ttagcattac gaggatgaag aagagggaga aagtgcatag
      601 catgtgcaga accacccggc agggccacaa cgaccgcttc aaccggaaat agcatgaatg
      661 tgaaaaacga caatagaggg gggaaacagc aagacctgtg ctgcaagcag tgcggcagat
      721 tctcccctct ctttgaatca gcgcgcccgc acccccaaaa cgagcagcaa ggaaatatac
      781 caacaaataa gatgaagaca tgtgaatgtg cgtcgcaaca acctcacaac tgggtaactg
      841 tttacggaaa acgaacttca aattatcctg acgaagtaaa gacccagcca cccaggcaac
      901 ctcctgaggc cactaaatac catagttgaa caggtgaggg cagcgatggt acaaaggtca
      961 gaagcttgat aaataaatag aagtgctttg ttgttgttgt tag
//
