LOCUS       sequence-81098-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RNAi-resistant mouse Sema4D with N-terminal myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: pCMV-myc-Sema4D RNAiR
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          146..242
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      303..312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccggactct agaggatccg gtactagagg
      121 aactgaaaaa ccagaaagtt aactggtaag tttagtcttt ttgtctttta tttcaggtcc
      181 cggatccggt ggtggtgcaa atcaaagaac tgctcctcag tggatgttgc ctttacttct
      241 aggcctgtac ggaagtgtta cttctgctct aaaagctgcg gaattgtacc cgcgggcccg
      301 ccgccaccat ggctgggatt ttctatttca tcctcttttc gtttctcttt ggaatttgcg
      361 acgctgtcac cggttctaga gggcccacca tggcatcaat gcagaagctg atctcagagg
      421 aggacctgct tatggccatg gaggcccgaa ttcggtcgac cgagatctct cgaggttcgg
      481 ccttgctgat gagtgaggac aaagacactc tgtatgtagg cgcccgggaa gcagtctttg
      541 cagtgaatgc gctgaacatc tctgagaagc aacatgaggt atattggaag gtctctgaag
      601 acaaaaaatc caagtgtgca gagaagggga aatcaaagca gacggagtgc cttaactaca
      661 ttcgagtact acagccacta agcagcactt ccctctatgt gtgtgggacc aatgcgttcc
      721 agcccacctg tgaccacctg aacttgacat ccttcaagtt tctggggaaa agtgaagatg
      781 gcaaaggaag atgccccttc gaccccgccc acagctacac atcagtcatg gttgggggcg
      841 agctctactc tgggacgtcc tataatttct tgggcagtga acccatcatc tctcgaaact
      901 cttcccacag tcccttgagg acggagtatg ccatcccgtg gctga
//
