LOCUS       sequence-81105-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA 1 against rat and mouse Sema4D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S
  TITLE     Sema4D localizes to synapses and regulates GABAergic synapse 
            development as a membrane-bound molecule in the mammalian 
            hippocampus.
  JOURNAL   Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10.
  PUBMED    24036351
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2013-11"; volume: "57:23-32. doi"; pages: " 
            10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10"
COMMENT     Sequence Label: pSuper 44-1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..65
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     49..65
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        85..299
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     220..239
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(372..388)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(373..389)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(417..435)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(456..472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(456..472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(469..491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    480..496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(504..510,511..528,529..534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    549..570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(687..704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc tagaactagt
       61 ggatcccccg ggctgcagga attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc
      121 gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct
      181 gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat
      241 caccataaac gtgaaatgtc tttggatttg ggaatcttat aagttctgta tgagaccaca
      301 gatccccagt ggacctcctt cctaaattca agagatttag gaaggaggtc cacttttttg
      361 gaaaagctta tcgataccgt cgacctcgag ggggggcccg gtaccagctt ttgttccctt
      421 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      481 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      541 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      601 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      661 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcagg
//
