LOCUS       sequence-81108-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated version of NLL-MetRS from endogenous MetG 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, 
            Schneewind O, Mazmanian SK, Tirrell DA
  TITLE     Identification of secreted bacterial proteins by noncanonical amino 
            acid tagging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 
            10.1073/pnas.1301740111. Epub 2013 Dec 17.
  PUBMED    24347637
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1301740111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-01-7- 7"; volume: "111"; issue: "1"; pages: "433-8"
COMMENT     Sequence Label: pAM1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..89
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      189..283
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat
       61 ttcacacaga attcattaaa gaggagaaat taactatgag aggatcgcat caccatcacc
      121 atcacggatc cgcatgcgag ctcggtaccc cgggtcgacc tgcagccaag cttaattagc
      181 tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt
      241 cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct agctctagag
      301 acgtccggcc ggagctccac cgcggtggcg gccgctctag agtcacttac ttaacatttt
      361 cccatttggt actatctaac cccttttcac tattaagaag taatgcctac tatgactcaa
      421 gtcgcgaaga aaattctggt gacgtgcgca aacccgtacg ctaacggctc aatccacctc
      481 ggccatatgc tggagcacat ccaggctgat gtctgggtcc gttaccagcg aatgcgcggc
      541 cacgaggtca acttcatctg cgccgacgat gcccacggta caccgatcat gctgaaagct
      601 cagcagcttg gtatcacccc ggagcagatg attggcgaaa tgagtcagga gcatcagact
      661 gatttcgcag gctttaacat cagctatgac aactatcact cgacgcacag cgaagagaac
      721 cgccagttgt cagaacttat ctactctcgc ctgaaagaaa acggttttat taaaaaccgc
      781 accatctctc agctgtacga tccggaaaaa ggcatgttcc tgccggaccg ttttgtgaaa
      841 ggcacctgcc cgaaatgtaa atccccggat caatacggcg ataactgcga agtctgcggc
      901 gcgacctaca
//
