LOCUS       sequence-81122-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a EGFP variant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-GFPmG1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..57)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(297..316)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     644..665
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
       61 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      121 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      181 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      241 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      301 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      361 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctgaagt
      421 acaactacca gagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      481 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      541 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      601 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      661 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gcggccgcac
      721 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      781 caaataccac tgagatct
//
