LOCUS       sequence-81124-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a GFP-dependent transcription factor component in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Tang JC, Szikra T, Kozorovitskiy Y, Teixiera M, Sabatini BL, Roska 
            B, Cepko CL
  TITLE     A nanobody-based system using fluorescent proteins as scaffolds for 
            cell-specific gene manipulation.
  JOURNAL   Cell. 2013 Aug 15;154(4):928-39. doi: 10.1016/j.cell.2013.07.021.
  PUBMED    23953120
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.07"; journalName: "Cell"; date: "2013-08-15- 
            15"; volume: "154"; issue: "4"; pages: "928-39"
COMMENT     Sequence Label: pCAG-rTetRDBD-GBP1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(169..510)
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYW
                     GQGTQVTVSS"
ORIGIN
        1 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
       61 ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag
      121 ccaccacctt ctgataggca gcctgcacct gaggagtgcg gccgcttaag aactaacagt
      181 cacttgtgtg ccctgtcccc agtattcaaa gcccacattg acgttggagt agtacacagc
      241 agtgtcctca ggtttcaggg agttcatctg gaggtacacg gtgtttctag catcgtccct
      301 actaatggtg aaccgtcctt tcacgctatc ctcgtaggaa ctcctatcgc cagcagatga
      361 cattccggcg acccattctc gctcttttcc aggtgcctgc ctgtaccatc tcatagagta
      421 ccgattcaca ggaaagccag aagcggcaca agacagtctc agtgatcccc caggctggac
      481 cagtgctcct ccggattcca cgagctgcac gtcggccatg ctagctccgc cgctctcgca
      541 cttcagctgc ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc
      601 ggcgccctgt ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt
      661 ggtgggtgtc tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc
      721 cagggtgaag tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg
      781 ctggcacagg aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt
      841 gcccaggtgc actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc
      901 gttgttcc
//
