LOCUS       sequence-81126-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned 
            into pCI-neo).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Iwashita Y, Fukuchi N, Waki M, Hayashi K, Tahira T
  TITLE     Genome-wide repression of NF-kappaB target genes by transcription 
            factor MIBP1 and its modulation by O-linked beta-N-acetylglucosamine
            (O-GlcNAc) transferase.
  JOURNAL   J Biol Chem. 2012 Mar 23;287(13):9887-900. doi: 
            10.1074/jbc.M111.298521. Epub 2012 Jan 31.
  PUBMED    22294689
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.298521"; journalName: "J Biol Chem"; date: 
            "2012-03-23- 23"; volume: "287"; issue: "13"; pages: "9887-900"
COMMENT     Sequence Label: pCI-neo-FHM
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..52
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagttcaatt acagctctta aggctagagt acttaatacg actcactata ggctagcctc
       61 gagatggact acaaggacga cgatgacaag ctcgatggag gataccccta cgacgtgccc
      121 gactacgccg acactgggga cacagctcta ggacaaaaag ctacctcaag gtctggagaa
      181 actgacagtg tgtcaggtag atggaggcag gaacaatcag ccggtcttaa gatgagcact
      241 ttcagcagtc aggaagggca gagacaaccg caaatagatc ctgaccagat cggaaatgta
      301 gcttcagctc agctgttcgg ttctgggaag ctggcctcgc ctggcgaggg cgtacatcaa
      361 gtcacagaga agcaataccc accacaccgt ccgagtccct acccatgcca acattcgctc
      421 tctttccctc agcactcatt gccccagggc atgatgcaca gcaacaagcc gcaccagagc
      481 ctcgagggcc ctccctggct cttctctggc cctttgccat ctgtggcctc tgaggactta
      541 tttccttttc caatgcacgg ccacagtagt ggttacccta gaaaaaagat ctcaagtctc
      601 aaccccgctt acagccaata ctcccagaaa agtatcgaac aggcagaaga tgctcacaaa
      661 aaagagcaca aacccaaaaa gccgggcaaa tacatctgcc cgtactgcag cagggcatgt
      721 gcaaaaccaa gtgtcctgaa gaaacacatc aggtcccata ccggtgagcg gccgtatccg
      781 tgtatacctt gtggtttctc tttcaagaca aagagcaatt tgtacaagca caggaagtct
      841 catgcccacg caattaaggc aggcttggta cccttcaccg aatcatctgt gtctaagctg
      901 gacctggagg ctggctttat tgatgtagaa gcagagatcc actcggatgg tgaacagagc
      961 acagacacgg atgaggagag ctctttattt gctgaggctt ctgacaaagt gagccccggc
     1021 cctcccatcc ctttggacat tgctagcaga ggtggctacc ccggatcctt ggaagaatcc
     1081 ttg
//
