LOCUS       sequence-81128-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PP1 alpha residues 7-330.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Kelker MS, Page R, Peti W
  TITLE     Crystal structures of protein phosphatase-1 bound to nodularin-R and
            tautomycin: a novel scaffold for structure-based drug design of 
            serine/threonine phosphatase inhibitors.
  JOURNAL   J Mol Biol. 2009 Jan 9. 385(1):11-21.
  PUBMED    18992256
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Jan 9. 385(1):11-21."
COMMENT     Sequence Label: RP1B-PP1alpha
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatatacc atgggctctg ataaaattca
       61 tcatcatcat catcacgaaa atctttattt tcaaggtcat atgggatccc tcaacctgga
      121 ctctatcatc gggcgcctgc tggaagtgca gggctcgcga cccggaaaga atgtgcagct
      181 gacggagaac gagatccgtg gtctgtgcct caaatcccgg gagatcttcc tgagccagcc
      241 cattctgctg gagctggagg cgcccctcaa gatctgcggt gacatccacg gccagtacta
      301 cgacctgctg cggctgttcg agtacggcgg cttcccccca gagagcaact acctgttcct
      361 gggtgactac gtggaccgcg gcaagcagtc cctggagacc atctgcctgc tgctggccta
      421 caagatcaag taccccgaga acttcttcct gctgcgcggg aaccacgagt gcgccagcat
      481 caaccgcatc tacggcttct acgacgagtg caagagacgc tacaacatca agctgtggaa
      541 gacgttcacc gactgcttca actgcctgcc catcgcggcc attgtggacg agaagatatt
      601 ctgctgccat ggcggcctct cccccgacct gcagtccatg gagcagatcc ggcgcatcat
      661 gcggcccacg gacgtgcccg accagggcct gctgtgtgac ctgctgtggt ctgaccccga
      721 caaggacgtg cagggctggg gcgagaacga ccgcggcgtc tccttcacct tcggcgcgga
      781 ggtggtggcc aagttcctgc acaagcatga cctggacctc atctgccggg cgcaccaggt
      841 ggtggaggac ggctatgagt tctttgccaa gcggcagctg gtgacacttt tctcagcccc
      901 caactactgt ggcgagttcg acaacgccgg agccatgatg agcgtggacg agaccctcat
      961 gtgctcctt
//
