LOCUS       sequence-81135-e        5385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti NPY-like receptor AAEL005310 (NPYLR4) full-length 
            clone in mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18ST-k5310
FEATURES             Location/Qualifiers
     source          1..5385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..362,363..631)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             363..631
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          661..757
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3288..3422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3325..3344)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3379..3398
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3461..3478)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3632..4221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3712..3731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4690..5068)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     5015..5034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5254..5358)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgatc
      361 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
      421 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
      481 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
      541 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
      601 tttcgttttc tgttctgcgc cgttacagat ccaagctctg aaaaaccaga aagttaactg
      661 gtaagtttag tctttttgtc ttttatttca ggtcccggat ccggtggtgg tgcaaatcaa
      721 agaactgctc ctcagtggtt gttgccttta cttctaggcc tgtacggaag tgttacttct
      781 gctctaaaag ctgcggaatt ccggaatttc ctcgaggcca ccatggcaag agcgatacca
      841 tcagcactag catcttcgca attattcctc ttggcactgt tcaccttcct gagtgtgggc
      901 gacacccagg aggacaacag tgctcaacag tccccgttgc accagctccg agtcaactcc
      961 taccgaccct ggtggacgcc aaggacacat aaccttctca tccccctgga cgaggatgcc
     1021 gtcagatggg cgaaccgatc tatggtttat cctagaacaa caaactcgtt aagttcagca
     1081 tcaggaccat cattgccagc aaccatcggt gtcctgaaaa acctatcatc gactccgacc
     1141 ggtcccagct tcgaaggtgg cccctctgga cccagactgg ctgttctggt gaagcgcgat
     1201 gtaattaaag tgcaagaatc tccattatta tcatcatcat catcatcgct atcgttgaag
     1261 tcgttgtcag cttcgacgat gagtgtgaga atcgaccggt ctttagaacc cagttccgag
     1321 ggaaagccac cagcgatgac gacgtcaaat tttaatggcg caatttgtcg tgacggaaac
     1381 aacgtcggga cagaggcgga gcaaggctcc ggaagctgtg ccctggtcaa caacggcact
     1441 aaatcacccc tcgccgcagg tctggacgga aaccagaccg tcgtcacgcc gtactacacc
     1501 atcgtcaacc tggacaacca taatgacgtc ctgtgcgacg aggagtacga caccgaggag
     1561 tacaacgaga actgtttcat cgatcataat gtgacctgcg tgggcgaccc cctgtactgc
     1621 aatcttacgt acgacgagta ccggcagcta ttgatggact atatctaccc gtcgacggcc
     1681 gaatggatcc tgattgcatc gcactcggtc gtgttcataa tggggctggt cggcaacgca
     1741 ctggtctgta tcgcagtcta caccaaccac tcgatgcgca ccgtgaccaa catcttcatc
     1801 gtgaacctgg cggtggcgga ctttttcgtg atcctgttct gccttccgcc gaccgtcgtc
     1861 tgggatgtga ccgaaacgtg gtttatgggc aaagccatgt gcaaggtcgt catctacttt
     1921 cagacggttt cggtgacggt atcggtcctg acgctgacat acatctcgat cgatcggtgg
     1981 tatgcgattt gctttcccct gcggtacaaa cctcggccgg aacgtgcctg gcggttcatt
     2041 gccgttatct ggttgattgg cttcctatca gaccttccgg agttcctggt gctgacgacc
     2101 cggcggaaaa aactacgctt cgacatcaag ctcttcactc agtgtgtgtc cacttgggac
     2161 aacgagaagg agaaaacctt ctacatcgtc aagttcgtgt tcctttactc gttaccgctt
     2221 ctgtttatga cgatagcgta ctttcaaatt gtacgagttc tgtggcggtc cgacaccatt
     2281 ccgggccatc gggaatcccg gacgcaaccc tacggcatcc acagcacccg gacgacgctg
     2341 aactgcgttg ggaacacgag caccatgggc cagctgcgag ctcgccggaa agcggccaag
     2401 atgttggtgg cggtcgttgt catgttcgcc agctgttact ttccggtgca catgctgaat
     2461 gtggccagat acacattcga catcgggcag agcgacgtgg tggccgtgct gtcgctgttt
     2521 tcccattggc tttgctacgc caacagcgcc gtcaatcctg tgatttataa ttttatgagt
     2581 ggcaagttcc gtcgggagtt caaaaatgcg ctggaaaagt gccactgcct gcggaatccg
     2641 cgagggctgg gaggacgcgt cggtggctac gatgaccgtt cgatgtacca cacggccacc
     2701 cggatgaatg ccagccccag cagtcgaagc aactaccacc tgaccagtgt aagaaacatt
     2761 agcattaagc acacgcagca gacatccttc aacaatggca gtcgccatca tcacgcccgg
     2821 aacagcatca atcatccggg cagtttgacc ggcgctccgc agatctcccc ggtgtccttc
     2881 gaggagcgga tggcccttac caagaacatg gacggcaaca ttggctgcgg tgaccccacc
     2941 atggcaggaa ctgccaccag tgttgccagt cgagcggaag gcaacagtag tggtcacgtt
     3001 ggcgccaaca gcaacaccaa tcatcatcat ctgcatcatc atggcgttgc gtgcaacgga
     3061 tcgacaccgg atgccccggc gaccaccacc ggaactgcac ctcccaccaa cggcggatcc
     3121 agttccatgc taatgattgt gaataaatcg agcaattgta agattaatgg cacttgacgc
     3181 gcgaagctgc acgtttcctg gcggcggccg ctagactagt ctagagaaaa aacctcccac
     3241 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3301 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3361 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3421 tccccgggta ccgagctcga attaattcct cttccgcttc ctcgctcact gactcgctgc
     3481 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3541 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     3841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4141 ccggcaaaca aaccaccgct ggtagcggtg ggtttttttg tttgcaagca gcagattacg
     4201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5101 ctgttgagat ccagttcgat gtaacccact cgtggcaccc aactgatctt cagcatcttt
     5161 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5221 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5281 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5341 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgc
//
